Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environmental and Operational Medicine, Tianjin 300050, People's Republic of China.
Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environmental and Operational Medicine, Tianjin 300050, People's Republic of China.
Sci Total Environ. 2023 Feb 1;858(Pt 2):159977. doi: 10.1016/j.scitotenv.2022.159977. Epub 2022 Nov 5.
A fluorescent biosensor strategy was developed in combination with immunomagnetic separation for rapid and sensitive detection of staphylococcal enterotoxin B (SEB). Magnetic nanoparticles (MNPs) modified with aptamer of SEB could capture the SEB. Then the gold nanoparticles (AuNPs) fluorescent probe was added and a "sandwich structure" was formed between AuNPs, SEB and MNPs. The MNPs-SEB-AuNPs structure could be separated with an additional magnetic field, which resulted the lower signals of AuNPs fluorescent probe. In optimal conditions, the current method displayed a broad quantitative range from 100 to 10 fg/mL and the limit of detection was 3.43 fg/mL. The recovery of SEB-spiked milk samples ranged from 92.00 to 119.00 %, which revealed that the developed method had great accuracy. Furthermore, the method was fast and economical for ultrasensitive detection. Therefore, the fluorescent biosensor based on MNPs-AuNPs is promising for the detection of other environmental and food pollutants.
建立了一种荧光生物传感器策略，结合免疫磁分离技术，用于快速灵敏地检测金黄色葡萄球菌肠毒素 B (SEB)。用 SEB 适体修饰的磁性纳米粒子 (MNPs) 可以捕获 SEB。然后加入金纳米粒子 (AuNPs) 荧光探针，在 AuNPs、SEB 和 MNPs 之间形成“三明治结构”。通过外加磁场可以分离 MNPs-SEB-AuNPs 结构，从而降低 AuNPs 荧光探针的信号。在最佳条件下，该方法的定量范围从 100 到 10 fg/mL，检测限为 3.43 fg/mL。SEB 加标牛奶样品的回收率在 92.00%至 119.00%之间，表明该方法具有很高的准确性。此外，该方法快速、经济，可用于超灵敏检测。因此，基于 MNPs-AuNPs 的荧光生物传感器有望用于检测其他环境和食品污染物。
