Peng Yuyang, Yin Huachun, Li Song, Yang Hui
Multidisciplinary Center for Pituitary Adenomas of Chongqing, Department of Neurosurgery, Xinqiao Hospital, Army Medical University, Chongqing, China.
Multidisciplinary Center for Pituitary Adenomas of Chongqing, Department of Neurosurgery, Xinqiao Hospital, Army Medical University, Chongqing, China; Chongqing Institute for Brain and Intelligence, Guangyang Bay Laboratory, Chongqing, China.
Genomics. 2022 Nov;114(6):110519. doi: 10.1016/j.ygeno.2022.110519. Epub 2022 Nov 5.
High altitude cerebral edema (HACE) is a serious subtype of acute mountain sickness (AMS). Studies have suggested that increased expression of corticotropin releasing hormone receptor 1 (CRFR1) in pituitary is related to the development of HACE, but no study has revealed the molecular landscape of pituitary function changes in this process. Rat model of HACE was established by simulating the high-altitude hypobaric hypoxia environment. Then RNA-sequencing was performed of rat pituitary gland (PG) in HACE and non-HACE groups. The function annotations, enrichment analysis, protein-protein interaction (PPI) network, chromosome location and drug repositioning of differentially expressed genes (DEGs) were explored based on the transcriptomic data. And we found pituitary secretion function was disordered in HACE, which was partly due to activated inflammation and oxidative stress. In addition, we identified potential biomarkers for early recognition of pituitary dysfunction and potential protective drugs for pituitary function in HACE.
高原脑水肿（HACE）是急性高原病（AMS）的一种严重亚型。研究表明，垂体中促肾上腺皮质激素释放激素受体1（CRFR1）表达增加与HACE的发生发展有关，但尚无研究揭示该过程中垂体功能变化的分子全貌。通过模拟高原低压低氧环境建立HACE大鼠模型。然后对HACE组和非HACE组大鼠的垂体（PG）进行RNA测序。基于转录组数据，对差异表达基因（DEG）进行功能注释、富集分析、蛋白质-蛋白质相互作用（PPI）网络、染色体定位及药物重定位研究。我们发现HACE中垂体分泌功能紊乱，部分原因是炎症和氧化应激激活。此外，我们还鉴定出了可早期识别垂体功能障碍的潜在生物标志物以及对HACE中垂体功能有潜在保护作用的药物。
