Department of Neurosurgery, The First Affiliated Hospital of Shantou University Medical College, Shantou, China.
Brain Behav. 2022 Dec;12(12):e2766. doi: 10.1002/brb3.2766. Epub 2022 Nov 9.
Patients with spontaneous subarachnoid hemorrhage (SAH) may develop refractory arterial cerebral vasospasm (CVS), which is the leading cause of death in SAH patients. This study explored the clinical diagnostic value of serum miR-195-5p levels in CVS after SAH (SAH + CVS) and its relationship with the prognosis of SAH + CVS.
A total of 110 patients with spontaneous SAH were divided into the SAH group (N = 62) and SAH + CVS group (N = 58), with 60 healthy subjects as controls. The clinical data of blood glucose, blood sodium fluctuation, and serum lactic acid were recorded. miR-195-5p serum level was detected by RT-qPCR and its diagnostic value on SAH + CVS was analyzed by receiver operating characteristic curve. Serum levels of PDGF/IL-6/ET-1 and their correlation with miR-195-5p were analyzed using RT-qPCR, enzyme-linked immunosorbent assay, and Pearson's method. The patient prognosis was evaluated by Glasgow Outcome Scale. The effect of miR-195-5p levels on adverse prognosis was analyzed by Kaplan-Meier method and Cox regression analysis.
miR-195-5p was lowly expressed in the serum of SAH patients and lower in SAH + CVS patients. Serum miR-195-5p level assisted the diagnosis of SAH and SAH + CVS and was negatively correlated with PDGF/IL-6/ET-1 levels and was an independent risk factor together with ET-1 and blood glucose for SAH + CVS. miR-195-5p low expression predicted a higher cumulative incidence of adverse outcomes and was an independent predictor of adverse outcomes.
Poor expression of miR-195-5p can assist the diagnosis of SAH + CVS and predict higher adverse outcomes.
自发性蛛网膜下腔出血（SAH）患者可能会出现难治性动脉性脑血管痉挛（CVS），这是 SAH 患者死亡的主要原因。本研究探讨了血清 miR-195-5p 水平在 SAH 后 CVS（SAH + CVS）中的临床诊断价值及其与 SAH + CVS 预后的关系。
共纳入 110 例自发性 SAH 患者，分为 SAH 组（N = 62）和 SAH + CVS 组（N = 58），另选取 60 例健康受试者作为对照组。记录血糖、血钠波动和血清乳酸等临床数据。采用 RT-qPCR 检测 miR-195-5p 血清水平，通过受试者工作特征曲线分析其对 SAH + CVS 的诊断价值。采用 RT-qPCR、酶联免疫吸附试验和 Pearson 法分析血清 PDGF/IL-6/ET-1 水平及其与 miR-195-5p 的相关性。采用 Glasgow 预后量表评估患者预后。采用 Kaplan-Meier 法和 Cox 回归分析 miR-195-5p 水平对不良预后的影响。
SAH 患者血清中 miR-195-5p 表达水平较低，SAH + CVS 患者更低。血清 miR-195-5p 水平有助于 SAH 和 SAH + CVS 的诊断，与 PDGF/IL-6/ET-1 水平呈负相关，与 ET-1 和血糖一起是 SAH + CVS 的独立危险因素。miR-195-5p 低表达预测不良结局的累积发生率较高，是不良结局的独立预测因子。
miR-195-5p 表达水平降低有助于诊断 SAH + CVS，并预测更高的不良结局。
