The Intercollege Graduate Degree Program in Integrative and Biomedical Physiology, Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, United States.
Department of Nutritional Sciences, The Pennsylvania State University, University Park, PA, United States; Division of Renal Diseases and Hypertension, University of Colorado Anschutz Medical Campus, Aurora, CO, United States.
J Nutr. 2024 May;154(5):1699-1710. doi: 10.1016/j.tjnut.2023.11.014. Epub 2023 Nov 19.
Proinflammatory cytokines are implicated in the pathophysiology of postmenopausal bone loss. Clinical studies demonstrate that prunes prevent bone mineral density loss; however, the mechanism underlying this effect is unknown.
We investigated the effect of prune supplementation on immune, inflammatory, and oxidative stress markers.
A secondary analysis was conducted in the Prune Study, a single-center, parallel-arm, 12-mo randomized controlled trial of postmenopausal women (55-75 y old; n = 235 recruited; n = 183 completed) who were assigned to 1 of 3 groups: "no-prune" control, 50 g prune/d and 100 g prune/d groups. At baseline and after 12 mo of intervention, blood samples were collected to measure serum high-sensitivity C-reactive protein (hs-CRP), serum total antioxidant capacity (TAC), plasma 8-isoprostane, proinflammatory cytokines [interleukin (IL)-1β, IL-6, IL-8, monocyte chemoattractant protein-1, and tumor necrosis factor (TNF)-α] concentrations in plasma and lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells (PBMCs) culture supernatants, and the percentage and activation of circulating monocytes, as secondary outcomes.
Prune supplementation did not alter hs-CRP, TAC, 8-isoprostane, and plasma cytokine concentrations. However, percent change from baseline in circulating activated monocytes was lower in the 100 g prune/d group compared with the control group (mean ± SD, -1.8% ± 4.0% in 100 g prune/d compared with 0.1% ± 2.9% in control; P < 0.01). Furthermore, in LPS-stimulated PBMC supernatants, the percent change from baseline in TNF-α secretion was lower in the 50 g prune/d group compared with the control group (-4.4% ± 43.0% in 50 g prune/d compared with 24.3% ± 70.7% in control; P < 0.01), and the percent change from baseline in IL-1β, IL-6, and IL-8 secretion was lower in the 100 g prune/d group compared with the control group (-8.9% ± 61.6%, -4.3% ± 75.3%, -14.3% ± 60.8% in 100 g prune/d compared with 46.9% ± 107.4%, 16.9% ± 70.6%, 39.8% ± 90.8% in control for IL-1β, IL-6, and IL-8, respectively; all P < 0.05).
Dietary supplementation with 50-100 g prunes for 12 mo reduced proinflammatory cytokine secretion from PBMCs and suppressed the circulating levels of activated monocytes in postmenopausal women. This trial was registered at clinicaltrials.gov as NCT02822378.
促炎细胞因子与绝经后骨丢失的病理生理学有关。临床研究表明，梅脯可预防骨密度流失；然而，其作用机制尚不清楚。
我们研究了梅脯补充对免疫、炎症和氧化应激标志物的影响。
对 Prune 研究进行了二次分析，这是一项针对绝经后女性（55-75 岁；招募了 235 人，183 人完成）的单中心、平行臂、为期 12 个月的随机对照试验，将其分为 3 组：“无梅脯”对照组、50 g 梅脯/d 组和 100 g 梅脯/d 组。在干预前和 12 个月后，采集血样以测量血清高敏 C 反应蛋白（hs-CRP）、血清总抗氧化能力（TAC）、血浆 8-异前列腺素、促炎细胞因子[白细胞介素（IL）-1β、IL-6、IL-8、单核细胞趋化蛋白-1 和肿瘤坏死因子（TNF）-α]在血浆和脂多糖（LPS）刺激的外周血单核细胞（PBMC）培养上清液中的浓度，以及循环单核细胞的百分比和激活作为次要结果。
梅脯补充剂并未改变 hs-CRP、TAC、8-异前列腺素和血浆细胞因子浓度。然而，与对照组相比，100 g 梅脯/d 组循环激活单核细胞的百分比从基线的变化较低（100 g 梅脯/d 组为-1.8%±4.0%，对照组为 0.1%±2.9%；P<0.01）。此外，在 LPS 刺激的 PBMC 上清液中，与对照组相比，50 g 梅脯/d 组 TNF-α分泌的百分比从基线的变化较低（50 g 梅脯/d 组为-4.4%±43.0%，对照组为 24.3%±70.7%；P<0.01），与对照组相比，100 g 梅脯/d 组的 IL-1β、IL-6 和 IL-8 分泌的百分比从基线的变化较低（100 g 梅脯/d 组为-8.9%±61.6%、-4.3%±75.3%、-14.3%±60.8%，对照组为 46.9%±107.4%、16.9%±70.6%、39.8%±90.8%，分别为 IL-1β、IL-6 和 IL-8；均 P<0.05）。
绝经后女性补充 50-100 g 梅脯 12 个月可减少 PBMC 中促炎细胞因子的分泌，并抑制循环中激活的单核细胞水平。该试验在 clinicaltrials.gov 上注册为 NCT02822378。
