State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology (Shandong academy of sciences), China.
Faculty of Light Industry, Qilu University of Technology (Shandong academy of sciences), China.
Org Biomol Chem. 2022 Nov 30;20(46):9184-9191. doi: 10.1039/d2ob01681h.
Recently, multifunctional fluorescent probes have received more and more attention with the rapid development of fluorescence imaging technology. In this article, a multifunctional fluorescent probe NAP-OH was designed and synthesized. NAP-OH exhibited outstanding polarity-sensing performance and was used to detect trace water in different organic solvents by means of fluorescence emission intensity. The fluorescence spectroscopy results indicated that NAP-OH was highly responsive to trace water in acetone (LOD = 28 ppm). The co-localization experiment and cell starvation imaging showed that the probe NAP-OH could be used to distinguish lysosomes from lipid droplets, showing bright red fluorescence in lipid droplets and weak red fluorescence in lysosomes. The oleic acid stimulation experiment shows that the probe can detect the dynamic changes of lipid droplets while observing lysosomes. In addition, NAP-OH showed great photostability and low cytotoxicity and was successfully used in zebrafish imaging. Therefore, these results indicated that NAP-OH, as a multifunctional fluorescent probe, would be used in trace water detection and biological imaging.
最近，随着荧光成像技术的飞速发展，多功能荧光探针受到了越来越多的关注。本文设计并合成了一种多功能荧光探针 NAP-OH。NAP-OH 表现出出色的极性传感性能，并通过荧光发射强度用于检测不同有机溶剂中的痕量水。荧光光谱结果表明，NAP-OH 对丙酮中的痕量水高度敏感（LOD = 28 ppm）。共定位实验和细胞饥饿成像表明，探针 NAP-OH 可用于区分溶酶体和脂滴，在脂滴中显示出明亮的红色荧光，在溶酶体中显示出较弱的红色荧光。油酸刺激实验表明，该探针可在观察溶酶体的同时检测脂滴的动态变化。此外，NAP-OH 表现出良好的光稳定性和低细胞毒性，并成功用于斑马鱼成像。因此，这些结果表明，NAP-OH 作为一种多功能荧光探针，将用于痕量水检测和生物成像。
