Liu Ming-Xuan, Xu Li, Zhu Peng-Fei, Li Xin, Shan Miao, Jin Wei, Chen Jing, Ling Yong, Zhang Xiao-Ling
School of Pharmacy, Nantong University, Nantong, 226001, China.
Institute of Translational Medicine, Medical College, Yangzhou University, Yangzhou, 225001, Jiangsu, China.
J Mater Chem B. 2023 Apr 5;11(14):3186-3194. doi: 10.1039/d2tb02621j.
Lipid droplets (LDs) and their autophagy by lysosomes are closely related to a variety of physiological and pathological conditions. Therefore, identifying and tracking LDs and the dynamic process of autophagy can provide useful information for the diagnostics and treatment of related diseases. However, few organic small molecule-based fluorescent probes can specifically recognize LDs and dynamically track their autophagy process. Herein, we synthesized a "discoloration" fluorescent bioprobe DPABP-BI with distinguishable features including red fluorescence emission (630 nm), large Stokes shift (145 nm), two-photon excitation and outstanding photostability and biocompatibility. In particular, LDs could be specifically identified the red fluorescence emission of DPABP-BI (colocalization constant of 0.98), while autophagolysosomes could be visualized the green fluorescence emission of its acid-hydrolyzed product (colocalization constant of 0.90) to track the autophagy dynamic process. In addition, DPABP-BI enabled the specific recognition of fatty substances in zebrafish larvae. In this study, a two-photon excited red light small molecule probe was constructed to identify LDs and track their autophagy dynamic process by changing the fluorescence emission wavelength.
脂滴（LDs）及其通过溶酶体的自噬与多种生理和病理状况密切相关。因此，识别和追踪脂滴以及自噬的动态过程可为相关疾病的诊断和治疗提供有用信息。然而，很少有基于有机小分子的荧光探针能够特异性识别脂滴并动态追踪其自噬过程。在此，我们合成了一种具有可区分特征的“变色”荧光生物探针DPABP-BI，包括红色荧光发射（630 nm）、大斯托克斯位移（145 nm）、双光子激发以及出色的光稳定性和生物相容性。特别地，脂滴可通过DPABP-BI的红色荧光发射被特异性识别（共定位常数为0.98），而自噬溶酶体可通过其酸水解产物的绿色荧光发射被可视化（共定位常数为0.90），以追踪自噬动态过程。此外，DPABP-BI能够特异性识别斑马鱼幼虫中的脂肪物质。在本研究中，构建了一种双光子激发的红光小分子探针，通过改变荧光发射波长来识别脂滴并追踪其自噬动态过程。
