Elango Dinakaran, Wang Wanyan, Thudi Mahender, Sebastiar Sheelamary, Ramadoss Bharathi Raja, Varshney Rajeev K
Department of Agronomy, Iowa State University, Ames, IA, United States.
Department of Plant Science, Penn State University, University Park, PA, United States.
Front Plant Sci. 2022 Oct 24;13:1024543. doi: 10.3389/fpls.2022.1024543. eCollection 2022.
Chickpea ( L.) is one of the major pulse crops, rich in protein, and widely consumed all over the world. Most legumes, including chickpeas, possess noticeable amounts of raffinose family oligosaccharides (RFOs) in their seeds. RFOs are seed oligosaccharides abundant in nature, which are non-digestible by humans and animals and cause flatulence and severe abdominal discomforts. So, this study aims to identify genetic factors associated with seed oligosaccharides in chickpea using the mini-core panel. We have quantified the RFOs (raffinose and stachyose), ciceritol, and sucrose contents in chickpea using high-performance liquid chromatography. A wide range of variations for the seed oligosaccharides was observed between the accessions: 0.16 to 15.13 mg g raffinose, 2.77 to 59.43 mg g stachyose, 4.36 to 90.65 mg g ciceritol, and 3.57 to 54.12 mg g for sucrose. Kabuli types showed desirable sugar profiles with high sucrose, whereas desi types had high concentrations RFOs. In total, 48 single nucleotide polymorphisms (SNPs) were identified for all the targeted sugar types, and nine genes (, and : ; and : ; : , and : ) were identified as potential candidate genes for sugar metabolism and transport in chickpea. The accessions with low RFOs and high sucrose contents may be utilized in breeding specialty chickpeas. The identified candidate genes could be exploited in marker-assisted breeding, genomic selection, and genetic engineering to improve the sugar profiles in legumes and other crop species.
鹰嘴豆（L.）是主要的豆类作物之一，富含蛋白质，在全球广泛食用。包括鹰嘴豆在内的大多数豆类种子中都含有大量棉子糖家族寡糖（RFOs）。RFOs是自然界中丰富的种子寡糖，人类和动物无法消化，会导致肠胃胀气和严重的腹部不适。因此，本研究旨在利用微型核心种质库鉴定与鹰嘴豆种子寡糖相关的遗传因素。我们使用高效液相色谱法对鹰嘴豆中的RFOs（棉子糖和水苏糖）、鹰嘴豆糖醇和蔗糖含量进行了定量分析。在不同种质之间观察到种子寡糖存在广泛的变异：棉子糖含量为0.16至15.13毫克/克，水苏糖含量为2.77至59.43毫克/克，鹰嘴豆糖醇含量为4.36至90.65毫克/克，蔗糖含量为3.57至54.12毫克/克。卡布利型鹰嘴豆呈现出理想的糖谱，蔗糖含量高，而迪西型鹰嘴豆的RFOs浓度高。总共为所有目标糖类鉴定出48个单核苷酸多态性（SNP），并鉴定出9个基因（，和：；和：；：，和：）作为鹰嘴豆糖代谢和转运的潜在候选基因。低RFOs和高蔗糖含量的种质可用于培育特色鹰嘴豆。所鉴定的候选基因可用于标记辅助育种、基因组选择和基因工程，以改善豆类和其他作物品种的糖谱。
