Haidoulis John Francis, Nicholson Paul
Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, England.
Front Plant Sci. 2022 Oct 24;13:1025161. doi: 10.3389/fpls.2022.1025161. eCollection 2022.
Fusarium head blight (FHB) and Fusarium root rot (FRR) are important diseases of small-grain cereals caused by species. While host response to FHB has been subject to extensive study, very little is known about response to FRR and the transcriptome responses of FHB and FRR have not been thoroughly compared. (Bd) is an effective model for investigating host responses to both FHB and FRR. In this study the transcriptome response of Bd to (Fg) infection of heads and roots was investigated. An RNA-seq analysis was performed on both Bd FHB and FRR during the early infection. Additionally, an RNA-seq analysis was performed on samples of Fg for comparison with Fg gene expression . Differential gene expression and gene-list enrichment analyses were used to compare FHB and FRR transcriptome responses in both Bd and Fg. Differential expression of selected genes was confirmed using RT-qPCR. Most genes associated with receptor signalling, cell-wall modification, oxidative stress metabolism, and cytokinin and auxin biosynthesis and signalling genes were generally upregulated in FHB or were downregulated in FRR. In contrast, Bd genes involved in jasmonic acid and ethylene biosynthesis and signalling, and antimicrobial production were similarly differentially expressed in both tissues in response to infection. A transcriptome analysis of predicted Fg effectors with the same infected material revealed elevated expression of core tissue-independent genes including cell-wall degradation enzymes and the gene cluster for DON production but also several tissue-dependent genes including those for aurofusarin production and cutin degradation. This evidence suggests that Fg modulates its transcriptome to different tissues of the same host.
赤霉病（FHB）和镰刀菌根腐病（FRR）是由镰刀菌属物种引起的小粒谷物的重要病害。虽然宿主对FHB的反应已得到广泛研究，但对FRR的反应了解甚少，且FHB和FRR的转录组反应尚未得到充分比较。二棱大麦（Bd）是研究宿主对FHB和FRR反应的有效模型。在本研究中，调查了Bd对禾谷镰刀菌（Fg）侵染穗部和根部的转录组反应。在早期感染期间，对Bd FHB和FRR均进行了RNA测序分析。此外，对Fg的样本进行了RNA测序分析，以与Fg基因表达进行比较。使用差异基因表达和基因列表富集分析来比较Bd和Fg中FHB和FRR的转录组反应。使用RT-qPCR确认了所选基因的差异表达。大多数与受体信号传导、细胞壁修饰、氧化应激代谢以及细胞分裂素和生长素生物合成及信号传导基因相关的基因在FHB中通常上调或在FRR中下调。相反，参与茉莉酸和乙烯生物合成及信号传导以及抗菌物质产生的Bd基因在两个组织中对感染的反应中同样存在差异表达。对相同感染材料进行的预测Fg效应子的转录组分析显示，核心组织非依赖性基因表达升高，包括细胞壁降解酶和脱氧雪腐镰刀菌烯醇（DON）产生的基因簇，但也有几个组织依赖性基因，包括金褐霉素产生和角质降解的基因。这一证据表明，Fg会将其转录组调节至同一宿主的不同组织。
