Xu Murong, Li Yutong, Tang Ying, Zhao Xiaotong, Xie Dandan, Chen Mingwei
Department of Endocrinology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230032, People's Republic of China.
Diabetes Metab Syndr Obes. 2022 Nov 3;15:3415-3428. doi: 10.2147/DMSO.S376292. eCollection 2022.
To investigate the correlations of miR-155 expression in the peripheral blood and wound margin tissue of patients with diabetic foot ulcer (DFU) and explore the clinical value of miR-155 as a potential biomarker for the diagnosis and treatment outcomes of DFU.
Sixty newly diagnosed T2DM patients without DFU (T2DM group), 112 T2DM patients with DFU (DFU group), and 60 healthy controls (NC group) were included. MiR-155 levels in the peripheral blood and wound margin tissue were determined by quantitative real-time PCR, while clinical features and risk factors of DFU were explored. Multiple stepwise logistic regression analysis was used to determine whether miR-155 expression was an independent risk factor for DFU. The diagnostic effectiveness of miR-155 level on DFU was evaluated using ROC curve analysis.
A significant decrease in the expression level of miR-155 was observed in T2DM group compared with NC group (P < 0.05), while a markedly increased miR-155 expression level was noted in DFU group compared with T2DM group (P < 0.01). Moreover, there was a negative correlation between the expression levels of miR-155 with healing rate of DFU. Kaplan-Meier survival curve analysis showed that the cumulative rate of unhealed DFU in miR-155 high expression group is higher than that in miR-155 low expression group, both in peripheral blood and wound margin tissue (log rank, P = 0.004, P < 0.001, respectively). The multivariate logistic regression analysis confirmed that a high expression of miR-155 was an independent risk factor for DFU. The ROC curve analysis indicated that the AUC of miR-155 for the diagnosis of DFU was 0.794, with the optimum sensitivity being 96.82% and the optimum specificity of 95.93%.
The increased expression of miR-155 in peripheral blood of T2DM patients is closely related to the occurrence of DFU. MiR-155 is a potentially valuable biomarker for diagnosis and prognosis of DFU.
探讨糖尿病足溃疡（DFU）患者外周血及创面边缘组织中miR-155表达的相关性，并探讨miR-155作为DFU诊断及治疗效果潜在生物标志物的临床价值。
纳入60例新诊断的无DFU的2型糖尿病患者（T2DM组）、112例DFU的2型糖尿病患者（DFU组）和60例健康对照者（NC组）。采用定量实时PCR测定外周血及创面边缘组织中miR-155水平，同时探讨DFU的临床特征及危险因素。采用多因素逐步logistic回归分析确定miR-155表达是否为DFU的独立危险因素。采用ROC曲线分析评估miR-155水平对DFU的诊断效能。
与NC组相比，T2DM组miR-155表达水平显著降低（P<0.05）；与T2DM组相比，DFU组miR-155表达水平显著升高（P<0.01）。此外，miR-155表达水平与DFU愈合率呈负相关。Kaplan-Meier生存曲线分析显示，外周血及创面边缘组织中miR-155高表达组DFU未愈合累积率均高于miR-155低表达组（对数秩检验，P值分别为0.004、<0.001）。多因素logistic回归分析证实，miR-155高表达是DFU发生的独立危险因素。ROC曲线分析提示，miR-155诊断DFU的AUC为0.794，最佳灵敏度为96.82%，最佳特异度为95.93%。
2型糖尿病患者外周血中miR-155表达升高与DFU的发生密切相关。miR-155是DFU诊断及预后的潜在有价值生物标志物。
