一种用于检测大量诱捕样本中番茄潜叶蛾（鳞翅目：麦蛾科）的液滴数字PCR（ddPCR）检测方法。

A Droplet Digital PCR (ddPCR) Assay to Detect Phthorimaea absoluta (Lepidoptera: Gelechiidae) in Bulk Trap Samples.

作者信息

Zink Frida A, Tembrock Luke R, Timm Alicia E, Gilligan Todd M

机构信息

Department of Agricultural Biology, Colorado State University, Fort Collins, CO 80523, USA.

USDA-APHIS-PPQ-Science & Technology, Pest Identification Technology Laboratory, Fort Collins, CO 80526, USA.

出版信息

J Econ Entomol. 2022 Dec 14;115(6):2125-2129. doi: 10.1093/jee/toac177.

DOI:10.1093/jee/toac177

PMID:36354050

Abstract

The moth species Phthorimaea absoluta (Meyrick) (formerly Tuta absoluta) is serious threat to tomato and other Solanaceous crops worldwide and is invasive throughout Europe, Asia, and Africa. While P. absoluta has not yet been found in the U.S. recent detections in the Caribbean have raised concerns that the species could be introduced to mainland North America. To improve detection capacity, a droplet digital PCR (ddPCR) assay was developed that employs a nondestructive bulk DNA extraction method able to detect one P. absoluta sample among 200 nontargets. Such high-throughput and sensitive molecular assays are essential to preventing introductions through early detection and response. This assay can also be used in areas where P. absoluta is established to monitor outbreaks and track migratory patterns.

摘要

番茄潜麦蛾（Phthorimaea absoluta (Meyrick)，原称番茄潜叶蛾Tuta absoluta）对全球番茄及其他茄科作物构成严重威胁，在欧洲、亚洲和非洲均有入侵。虽然美国尚未发现番茄潜麦蛾，但最近在加勒比地区的检测引发了人们对该物种可能被引入北美大陆的担忧。为提高检测能力，开发了一种液滴数字PCR（ddPCR）检测方法，该方法采用一种非破坏性的大量DNA提取方法，能够在200个非目标样本中检测出一个番茄潜麦蛾样本。这种高通量且灵敏的分子检测方法对于通过早期检测和应对来防止物种引入至关重要。该检测方法还可用于番茄潜麦蛾已定殖的地区，以监测疫情爆发并追踪其迁飞模式。