Zhejiang Laboratory, Research Center for Intelligent Sensing Systems, Hangzhou 311121, China.
Biosensors (Basel). 2022 Oct 24;12(11):917. doi: 10.3390/bios12110917.
Hypocalcemia is a disease that adversely affects the production and reproduction of dairy cows. A portable device for rapid bovine blood calcium sensing has been growing in demand. Herein, we report a smartphone-based ratiometric fluorescence probe (SRFP) platform as a new way to detect and quantify calcium ions (Ca) in blood serum. Specifically, we employed a cost-effective and portable smartphone-based platform coupled with customized software that evaluates the response of Ca ions to ratiometric fluorescence probe in bovine serum. The platform consists of a three-dimensional (3D) printed housing and low-cost optical components that excite fluorescent probe and selectively transmit fluorescence emissions to smartphones. The customized software is equipped with a calibration model to quantify the acquired fluorescence images and quantify the concentration of Ca ions. The ratio of the green channel to the red channel bears a highly reproducible relationship with Ca ions concentration from 10 μM to 40 μM in bovine serum. Our detection system has a limit of detection (LOD) of 1.8 μM in bovine serum samples and the recoveries of real samples ranged from 92.8% to 110.1%, with relative standard deviation (RSD) ranging from 1.72% to 4.89%. The low-cost SRFP platform has the potential to enable campesino to rapidly detect Ca ions content in bovine serum on-demand in any environmental setting.
低钙血症是一种会对奶牛的生产和繁殖造成负面影响的疾病。一种用于快速检测牛血液中钙的便携式设备的需求日益增长。在此，我们报告了一种基于智能手机的比率荧光探针（SRFP）平台，作为一种新的方法来检测和定量血清中的钙离子（Ca）。具体来说，我们采用了一种具有成本效益和便携性的基于智能手机的平台，结合了自定义软件，该软件评估了 Ca 离子对牛血清中比率荧光探针的响应。该平台由 3D 打印外壳和低成本光学元件组成，这些光学元件可激发荧光探针，并选择性地将荧光发射传输到智能手机。定制软件配备了一个校准模型，用于定量获取的荧光图像并定量 Ca 离子的浓度。绿色通道与红色通道的比值与牛血清中从 10 μM 到 40 μM 的 Ca 离子浓度具有高度可重现的关系。我们的检测系统在牛血清样品中的检测限（LOD）为 1.8 μM，实际样品的回收率范围为 92.8%至 110.1%，相对标准偏差（RSD）范围为 1.72%至 4.89%。低成本的 SRFP 平台有可能使农民能够在任何环境条件下按需快速检测牛血清中的 Ca 离子含量。
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