School of Chemistry and Molecular Engineering, Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, China.
School of Chemistry and Molecular Engineering, Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, China.
Anal Chim Acta. 2022 Mar 15;1198:339572. doi: 10.1016/j.aca.2022.339572. Epub 2022 Feb 2.
The abuse of the antibiotic tetracycline (TC) has resulted in its residues in the environment and animals, which has caused irreversible damage to public health safety and environments. Therefore, it is urgently needed to develop alternative sensitive and low-cost for quickly and accurately detection the TC residues in the environments. We innovatively rational design of MoS QDs and Eu to construct ratiometric fluorescent probe to detect TC. In general, TC coordinates Eu to form Eu-TC coordination compound with weak luminescence. However, MoS QDs with exceptional fluorescence characteristics are a good energy donors and acceptors, MoS QDs can transfer energy with Eu-TC coordination compound, which can greatly enhance the luminous ability of Eu-TC coordination compound while accompanied by a decline of its initial fluorescence intensity. MoS QDs can be used as an indicator and enhancer in the MoS-Eu ratiometric probe, which can greatly improve limit of detection and accuracy of quantitation for TC. The detection of TC by fluorescent intensity ratio (F/F) of MoS QDs-Eu shows a good linear response in the range of 10 nM-60 μM TC, with the limit of detection (LOD) toward TC is calculated to be 2 nM (3σ/slope), the recovery is 94.4-108.4%, and the RSD is less than 5.36. The smartphone-based portable platform with the RGB color recognition software is designed for point-of-care visual quantitative detection of TC. The portable detection system shows a low detection limit (0.05 μM) and excellent reproducibility in the range of 1-40 μM TC. This work has provided a sensitive, rapid, real-time detection of environmental pollutants.
抗生素四环素（TC）的滥用导致其在环境和动物中残留，对公共卫生安全和环境造成了不可逆转的损害。因此，迫切需要开发替代的、敏感的和低成本的方法，以快速、准确地检测环境中的 TC 残留。我们创新性地合理设计了 MoS QDs 和 Eu 来构建比率荧光探针来检测 TC。通常，TC 与 Eu 配位形成发光较弱的 Eu-TC 配合物。然而，具有优异荧光特性的 MoS QDs 是良好的能量供体和受体，MoS QDs 可以与 Eu-TC 配合物进行能量转移，从而极大地增强 Eu-TC 配合物的发光能力，同时伴随其初始荧光强度的下降。MoS QDs 可以用作 MoS-Eu 比率探针中的指示剂和增强剂，极大地提高了 TC 的检测限和定量精度。MoS QDs-Eu 的荧光强度比（F/F）检测 TC 呈现出 10 nM-60 μM TC 范围内的良好线性响应，对 TC 的检测限（LOD）计算为 2 nM（3σ/斜率），回收率为 94.4-108.4%，RSD 小于 5.36%。设计了基于智能手机的带有 RGB 颜色识别软件的便携式平台，用于现场可视化定量检测 TC。便携式检测系统在 1-40 μM TC 范围内表现出低检测限（0.05 μM）和优异的重现性。这项工作为环境污染物的敏感、快速、实时检测提供了一种新方法。
