Department of Biomedical Engineering, College of Life Science and Biotechnology, Dongguk University, Seoul 04620, Korea.
Department of BioNano Technology, Gachon University, Seongnam 13120, Gyeonggi, Korea.
Biosensors (Basel). 2022 Nov 7;12(11):979. doi: 10.3390/bios12110979.
Nucleic acid aptamer-based research has focused on achieving the highest performance for bioassays. However, there are limitations in evaluating the affinity for the target analytes in these nucleic acid aptamer-based bioassays. In this study, we mainly propose graphene oxide (GO)-based electrical and optical analyses to efficiently evaluate the affinity between an aptamer and its target. We found that an aptamer-coupled GO-based chip with an electrical resistance induced by a field-effect transistor, with aptamers as low as 100 pM, can detect the target, thrombin, at yields as low as 250 pM within five minutes. In the optical approach, the fluorescent dye-linked aptamer, as low as 100 nM, was efficiently used with GO, enabling the sensitive detection of thrombin at yields as low as 5 nM. The cantilever type of mechanical analysis also demonstrated the intuitive aptamer-thrombin reaction in the signal using dBm units. Finally, a comparison of electrical and optical sensors' characteristics was introduced in the attachment and detachment of aptamer to propose an efficient analysis that can be utilized for various aptamer-based research fields.
基于核酸适体的研究集中于实现生物测定的最高性能。然而，在评估这些基于核酸适体的生物测定中对目标分析物的亲和力方面存在限制。在这项研究中，我们主要提出基于氧化石墨烯（GO）的电学和光学分析，以有效地评估适体与其靶标的亲和力。我们发现，基于适体偶联的 GO 芯片具有由场效应晶体管引起的电阻变化，可以在五分钟内以低至 250 pM 的产量检测到靶标凝血酶，其适体浓度低至 100 pM。在光学方法中，荧光染料偶联的适体，低至 100 nM，与 GO 有效地结合，能够以低至 5 nM 的产量灵敏地检测到凝血酶。悬臂式机械分析也使用 dBm 单位展示了信号中直观的适体-凝血酶反应。最后，介绍了电传感器和光传感器特性的比较，以提出一种可用于各种基于适体的研究领域的有效分析方法。
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