Gao Zhuo, Wang Pan, Chen Chang, Duan Jian, Feng Shilun, Liu Bo
School of Microelectronics, Shanghai University, Shanghai 201800, China.
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.
Micromachines (Basel). 2025 May 15;16(5):581. doi: 10.3390/mi16050581.
The increasing spread of infectious diseases caused by pathogenic microorganisms underscores the urgent need for highly sensitive, portable, and rapid nucleic acid detection technologies to facilitate early diagnosis and effective prevention. In this study, we developed a fluorescence-based nucleic acid detection platform that integrates a microfluidic chip with an all-inorganic perovskite photodetector. The system enables integrated operation of nucleic acid extraction, purification, and amplification on a microfluidic chip, combined with real-time electrical signal readout via a CsPbBr perovskite photodetector. Experimental results indicate that the photodetector exhibits high responsivity at 530 nm, aligning well with the primary emission peak of FAM. The system demonstrates a strong linear correlation between photocurrent and FAM concentration over the range of 0.01-0.4 μM (R = 0.928), with a low detection limit of 0.01 μM and excellent reproducibility across multiple measurements. Validation using FAM standard solutions and Escherichia coli samples confirmed the system's reliable linearity and signal stability. This platform demonstrates strong potential for rapid pathogen screening and point-of-care diagnostic applications.
致病性微生物引起的传染病传播日益广泛，凸显了对高灵敏度、便携式和快速核酸检测技术的迫切需求，以促进早期诊断和有效预防。在本研究中，我们开发了一种基于荧光的核酸检测平台，该平台将微流控芯片与全无机钙钛矿光电探测器集成在一起。该系统能够在微流控芯片上实现核酸提取、纯化和扩增的集成操作，并通过CsPbBr钙钛矿光电探测器进行实时电信号读出。实验结果表明，该光电探测器在530nm处具有高响应度，与FAM的主要发射峰良好匹配。该系统在0.01-0.4μM范围内光电流与FAM浓度之间呈现出很强的线性相关性（R = 0.928），检测限低至0.01μM，多次测量具有出色的重现性。使用FAM标准溶液和大肠杆菌样本进行的验证证实了该系统可靠的线性和信号稳定性。该平台在快速病原体筛查和即时诊断应用方面显示出强大的潜力。
