Joo Hyun Woo, Kim Min Kyu, Bak Soon Sun, Sung Young Kwan
Department of Immunology, School of Medicine, Kyungpook National University, Daegu 41944, Korea.
BK21 FOUR KNU Convergence Educational Program of Biomedical Sciences for Creative Future Talents, Department of Biomedical Sciences, School of Medicine, Kyungpook National University, Daegu 41944, Korea.
Bioengineering (Basel). 2022 Nov 3;9(11):645. doi: 10.3390/bioengineering9110645.
We aimed to establish screening and efficacy test techniques for use in the development of hair-promoting agents. To this end, we used the dermal papilla cell (DPc)-derived immortalized cell line (SV40T-hTERT DPc) and neonatal foreskin-derived keratinocyte cell line (Ker-CT) to form an immortalized cell-based hair follicle-like structure. The SV40T-hTERT DPc spheroids exhibited a higher cell ratio in the spheroids than primary DPc spheroids, and SV40T-hTERT DPc aggregated with spheroids larger in diameter than primary DPc when the same cell number was seeded into the low-adhesion plate. Microscopic imaging and fluorescence staining results indicated that both primary and immortalized cell combinations form a hair follicle-like structure with a long-stretched keratinocyte layer under the condition that the spheroids have the same diameter as that of in vivo dermal papillary tissue in the hair follicle. The hair follicle-like structure elongation was increased upon treatment with three known hair follicle growth-promoting compounds (minoxidil, tofacitinib, and ascorbic acid) compared with that in the control group. Therefore, using immortalized cells to generate a coherent follicle-like structure, we have developed models for screening and evaluating hair-care materials commonly used in the industry.
我们旨在建立用于生发剂开发的筛选和功效测试技术。为此，我们使用真皮乳头细胞（DPc）来源的永生化细胞系（SV40T-hTERT DPc）和新生儿包皮来源的角质形成细胞系（Ker-CT）来形成基于永生化细胞的毛囊样结构。当将相同数量的细胞接种到低粘附板中时，SV40T-hTERT DPc球体在球体中的细胞比例高于原代DPc球体，并且SV40T-hTERT DPc与直径比原代DPc更大的球体聚集。显微镜成像和荧光染色结果表明，在球体直径与毛囊中体内真皮乳头组织直径相同的条件下，原代细胞和永生化细胞组合均形成具有长拉伸角质形成细胞层的毛囊样结构。与对照组相比，用三种已知的促进毛囊生长的化合物（米诺地尔、托法替布和抗坏血酸）处理后，毛囊样结构的伸长增加。因此，通过使用永生化细胞生成连贯的毛囊样结构，我们开发了用于筛选和评估该行业常用护发材料的模型。
