Anđelović Sara, Božinović Marko, Ćurić Željka, Šalić Anita, Jurinjak Tušek Ana, Zagajski Kučan Kristina, Rogošić Marko, Radović Mia, Cvjetko Bubalo Marina, Zelić Bruno
Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, HR-10000 Zagreb, Croatia.
Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva ul. 6, HR-10000 Zagreb, Croatia.
Bioengineering (Basel). 2022 Nov 8;9(11):665. doi: 10.3390/bioengineering9110665.
The most important and commonly used process for biodiesel synthesis is transesterification. The main by-product of biodiesel synthesis by transesterification is glycerol, which must be removed from the final product. Recently, deep eutectic solvent (DES) assisted extraction has been shown to be an effective and sustainable method for biodiesel purification. In this study, biodiesel was produced by lipase-catalysed transesterification from sunflower oil and methanol. A total of 12 different eutectic solvents were prepared and their physical properties were determined. Mathematical models were used to define which physical and chemical properties of DES and to what extent affect the efficiency of extraction of glycerol from the biodiesel. After initial screening, cholinium-based DES with ethylene glycol as hydrogen bond donor was selected and used for optimization of extraction process conditions performed in a microsystem. To determine the optimal process conditions (temperature, biodiesel:DES volume ratio, residence time), the experimental three-level-three-factor Box-Behnken experimental design was used. In the end, a combination of a mathematical model and experimental results was used to estimate how many micro-extractors are necessary for the complete removal of glycerol.
生物柴油合成最重要且最常用的方法是酯交换反应。通过酯交换反应合成生物柴油的主要副产物是甘油，必须从最终产物中去除。最近，深共熔溶剂（DES）辅助萃取已被证明是一种用于生物柴油提纯的有效且可持续的方法。在本研究中，通过脂肪酶催化向日葵油与甲醇的酯交换反应来生产生物柴油。总共制备了12种不同的共熔溶剂，并测定了它们的物理性质。使用数学模型来确定DES的哪些物理和化学性质以及在何种程度上影响从生物柴油中萃取甘油的效率。经过初步筛选，选择了以乙二醇作为氢键供体的胆碱基DES，并用于在微系统中进行的萃取工艺条件优化。为了确定最佳工艺条件（温度、生物柴油：DES体积比、停留时间），采用了实验性的三水平三因素Box-Behnken实验设计。最后，结合数学模型和实验结果来估计完全去除甘油需要多少个微萃取器。
