家蚕Abl1通过谷胱甘肽代谢调控丝蛋白合成

BmAbl1 Regulates Silk Protein Synthesis via Glutathione Metabolism in .

作者信息

Qin Sheng, Sun Lingling, Zhang Shu, Sun Xia, Li Muwang

机构信息

Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China.

Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture and Rural Affairs, Sericultural Research Institute, Chinese Academy of Agricultural Science, Zhenjiang 212100, China.

出版信息

Insects. 2022 Oct 22;13(11):967. doi: 10.3390/insects13110967.

DOI:10.3390/insects13110967

PMID:36354792

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9696079/

Abstract

, domesticated from wild silkworms, is an economic insect that feeds on mulberry leaves and produces silk. In the current study, we demonstrated the contribution of in silk protein synthesis. The inhibition and knockout of can reduce the larva weight and CSW. The effect on CSW of is not on the transcriptional level, but on the translational level. RNA-sequencing data suggested that amino acid synthesis and the metabolism process had a great difference between the and Control strain, particularly glutathione metabolism. An abnormality in glutathione metabolism led to the reduction of free glycine and serine content, which are the main components of fibroin protein. Finally, fibroin protein synthesis has been reduced, including fibroin-heavy chain, fibroin-light chain, and p25 protein. This finding brought to light the role of in the silk protein synthesis process.

摘要

家蚕由野蚕驯化而来，是一种以桑叶为食并产丝的经济昆虫。在本研究中，我们证明了（此处原文缺失关键信息）在丝蛋白合成中的作用。（此处原文缺失关键信息）的抑制和敲除会降低幼虫体重和茧丝产量。（此处原文缺失关键信息）对茧丝产量的影响不是在转录水平，而是在翻译水平。RNA测序数据表明，（此处原文缺失关键信息）与对照品系之间氨基酸合成和代谢过程存在很大差异，尤其是谷胱甘肽代谢。谷胱甘肽代谢异常导致丝素蛋白主要成分游离甘氨酸和丝氨酸含量降低。最后，丝素蛋白合成减少，包括丝素重链、丝素轻链和p25蛋白。这一发现揭示了（此处原文缺失关键信息）在丝蛋白合成过程中的作用。