Sun Chao, Shabbir Samina, Wang Wenxiang, Gao Yan, Ge Cuicui, Lin Qingsheng
The Key Laboratory of Natural Products of Guizhou Province and Chinese Academy of Sciences, Guizhou Medical University (GMU), Guiyang 550000, China.
Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Insects. 2022 Nov 3;13(11):1017. doi: 10.3390/insects13111017.
The oriental fruit fly is a polyphagous and highly invasive economically important pest in the world. We proposed the hypothesis that radiation treatment influence RNA expression in the larvae and leads to emergence failure. Therefore, transcriptome analyses of third-instar larvae of B. dorsalis ionizing, irradiated with 60Co-γ at 116Gy, were conducted and compared with the controls; a total of 608 DEGs were identified, including 348 up-regulated genes and 260 down-regulated ones. In addition, 130 SNPs in 125 unigenes were identified. For the DEGs, the most significantly enriched GO item was hemolymph coagulation, and some of the enriched pathways were involved in digestive processes. The subsequent validation experiment confirmed the differential expression of six genes, including sqd, ENPEP, Jhe, mth, Notch, and Ugt. Additionally, the 3401:G->A SNP in the Notch gene was also successfully validated. According to previous research, this was the first comparative transcriptome study to discover the candidate genes involved in insect molt to pupae. These results not only deepen our understanding of the emerging mechanism of B. dorsalis but also provide new insights into the research of biomarkers for quarantine insect treatment with the appropriate dose of radiation.
东方果实蝇是一种多食性且极具入侵性的世界性重要经济害虫。我们提出了辐射处理会影响幼虫RNA表达并导致羽化失败的假说。因此，对用116Gy的60Co-γ进行电离辐射的桔小实蝇三龄幼虫进行了转录组分析，并与对照组进行比较；共鉴定出608个差异表达基因（DEG），包括348个上调基因和260个下调基因。此外，在125个单基因中鉴定出130个单核苷酸多态性（SNP）。对于差异表达基因，最显著富集的基因本体（GO）项是血淋巴凝固，一些富集的途径涉及消化过程。后续的验证实验证实了六个基因的差异表达，包括sqd、ENPEP、Jhe、mth、Notch和Ugt。此外，Notch基因中的3401:G->A单核苷酸多态性也得到了成功验证。根据以往的研究，这是首次通过比较转录组研究发现参与昆虫蜕皮至蛹期的候选基因。这些结果不仅加深了我们对桔小实蝇羽化机制的理解，也为以适当剂量辐射处理检疫昆虫的生物标志物研究提供了新的见解。
