Zang Lian-Sheng, Akhtar Zunnu Raen, Ali Asad, Tariq Kaleem, Campos Mateus R
Key Laboratory of Green Pesticide and Agricultural Bioengineering, Guizhou University, Guiyang 550025, China.
Department of Entomology, University of Agriculture Faisalabad, Faisalabad 38000, Pakistan.
Insects. 2022 Nov 5;13(11):1023. doi: 10.3390/insects13111023.
Tomato pinworm, (Meyrick) (Lepidoptera: Gelechiidae) is the major pest of tomato crops in Pakistan. Insecticides are commonly used for the management of this insect-pest. To develop a better insecticide resistance management strategy and evaluate the risk of resistance evolution, a field collected population of the tomato pinworm was selected with flubendiamide in the laboratory. We investigated the genetics of flubendiamide resistance and concentration-mortality response to other insecticides by selecting a field strain of tomato pinworm with commercial flubendiamide formulation. was reciprocally crossed with resistant strain (Fluben-R) and was selected up to 13 generations, while F progeny was back-crossed with resistant parent (Fluben-R). The results of LC and Resistance Ratio (RR) demonstrated a higher resistance developed in field and laboratory-selected strains (G and G, respectively). Field-collected and laboratory-selected (Fluben-R) strains demonstrated higher intensity of concentration-mortality response against chlorantraniliprole, thiamethoxam, permethrin, abamectin and tebufenozide compared to susceptible ones. Based on the overlapping of 95% FL, it demonstrated significant differences, revealing that it was not sex linked (autosomal) with no maternal effects. The backcross analysis of the F´ resistant parent resulting in significant differences at all concentrations suggests that resistance is controlled by more than one factor; the null hypothesis was rejected and inheritance was under polygenic control. Resistance progression from 38 to 550 folds demonstrated that can develop a higher level of resistance to flubendiamide. Concentration-mortality response experiments demonstrated that the LC of some tested insecticides was higher for field-collected and laboratory-selected strains, suggesting that resistance mechanisms should be studied at a molecular level for better understanding. These results could be helpful to design resistance management strategies against the tomato pinworm.
番茄潜叶蛾（Meyrick）（鳞翅目：麦蛾科）是巴基斯坦番茄作物的主要害虫。杀虫剂常用于防治这种害虫。为了制定更好的抗杀虫剂管理策略并评估抗性进化的风险，在实验室中用氟苯虫酰胺对田间采集的番茄潜叶蛾种群进行了筛选。我们通过用市售氟苯虫酰胺制剂筛选番茄潜叶蛾的田间品系，研究了对氟苯虫酰胺抗性的遗传学以及对其他杀虫剂的浓度-死亡率反应。将其与抗性品系（氟苯虫酰胺-R）进行正反交，并连续筛选13代，而F1代子代与抗性亲本（氟苯虫酰胺-R）回交。LC50和抗性比率（RR）的结果表明，田间和实验室筛选的品系（分别为G1和G2）产生了更高的抗性。与敏感品系相比，田间采集和实验室筛选的（氟苯虫酰胺-R）品系对氯虫苯甲酰胺、噻虫嗪、氯菊酯、阿维菌素和虫酰肼表现出更高强度的浓度-死亡率反应。基于95%置信限的重叠，表明存在显著差异，这表明抗性不是伴性遗传（常染色体遗传），也没有母系效应。F1代与抗性亲本的回交分析在所有浓度下均产生显著差异，表明抗性受多个因素控制；原假设被拒绝，遗传受多基因控制。抗性从38倍提高到550倍表明番茄潜叶蛾能够对氟苯虫酰胺产生较高水平的抗性。浓度-死亡率反应实验表明，一些测试杀虫剂的LC50对于田间采集和实验室筛选的品系更高，这表明应在分子水平上研究抗性机制，以便更好地理解。这些结果有助于设计针对番茄潜叶蛾的抗性管理策略。
