Roditakis Emmanouil, Mavridis Konstantinos, Riga Maria, Vasakis Emmanouil, Morou Evangelia, Rison Jean Luc, Vontas John
Hellenic Agricultural Organisation - 'Demeter', Institute of Olive, Subtropical Plants and Vine, Heraklion, Crete, Greece.
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Crete, Greece.
Pest Manag Sci. 2017 Aug;73(8):1679-1688. doi: 10.1002/ps.4513. Epub 2017 Feb 20.
Indoxacarb is an important active ingredient extensively used for the control of Tuta absoluta, a major tomato pest, playing a particular role in insecticide resistance management schemes.
Reduced susceptibility to indoxacarb was identified (1794-fold resistance) through toxicological bioassays in a field population from Greece and evolved rapidly to resistance after short laboratory selection. Combined bioassays with synergists and biochemical analysis suggested only a partial involvement of detoxification enzymes in the resistant phenotype. To investigate the role of target-site resistance, segment 6 of domain IV of the sodium channel in T. absoluta was cloned and the sequences compared between susceptible and indoxacarb-resistant T. absoluta insects. The presence of the F1845Y and the V1848I indoxacarb resistance mutations was detected and was strongly associated with the phenotype. These amino acid substitutions correspond to recently characterised indoxacarb resistance mutations in diamondback moth (Plutella xylostella). Robust and accurate PCR-RFLP assays were subsequently developed and successfully validated for detecting both indoxacarb resistance mutations in field T. absoluta populations.
The identification of indoxacarb resistance mutations and the development of diagnostic tools will allow early detection of indoxacarb resistance, facilitating implementation of appropriate resistance management strategies, thus delaying the spread of resistance. © 2016 Society of Chemical Industry.
茚虫威是一种重要的活性成分，被广泛用于防治番茄主要害虫番茄潜叶蛾，在杀虫剂抗性管理方案中发挥着特殊作用。
通过对希腊一个田间种群进行毒理学生物测定，发现对茚虫威的敏感性降低（抗性倍数为1794倍），并且在实验室短期筛选后抗性迅速进化。与增效剂的联合生物测定和生化分析表明，解毒酶仅部分参与了抗性表型。为了研究靶标位点抗性的作用，克隆了番茄潜叶蛾钠通道结构域IV的第6段，并比较了敏感和茚虫威抗性番茄潜叶蛾昆虫之间的序列。检测到F1845Y和V1848I茚虫威抗性突变的存在，并且与表型密切相关。这些氨基酸取代对应于小菜蛾中最近鉴定出的茚虫威抗性突变。随后开发并成功验证了强大而准确的PCR-RFLP测定法，用于检测田间番茄潜叶蛾种群中的两种茚虫威抗性突变。
茚虫威抗性突变的鉴定和诊断工具的开发将有助于早期检测茚虫威抗性，促进实施适当的抗性管理策略，从而延缓抗性的传播。©2016化学工业协会。
