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牛指甲膜作为感染菌种的甲癣体外模型的有效性。

Efficacy of Bovine Nail Membranes as In Vitro Model for Onychomycosis Infected by Species.

作者信息

Temporiti Marta Elisabetta Eleonora, Guerini Marta, Baiguera Rebecca Michela, Buratti Simone, Desiderio Anthea, Goppa Lorenzo, Perugini Paola, Savino Elena

机构信息

Department of Earth and Environmental Sciences (DSTA), University of Pavia, Via Sant'Epifanio 14, 27100 Pavia, Italy.

Department of Drug Sciences, University of Pavia, Via Taramelli 12-14, 27100 Pavia, Italy.

出版信息

J Fungi (Basel). 2022 Oct 27;8(11):1133. doi: 10.3390/jof8111133.

DOI:10.3390/jof8111133
PMID:36354900
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9695884/
Abstract

Onychomycosis is a fungal infection caused by different etiologic agents, including dermatophytes that specifically colonize keratin-rich substrates. The aim of this work was to investigate mechanical modifications of bovine membranes (used as an in vitro nail model) placed in contact with species. strains were isolated from toenails specimens. The procedure was set up by spreading and strains on Petri dishes with minimal and rich media; after that, bovine membranes were placed in the center. After 27 days, and significantly reduced the thickness of the colonized membranes, whereas two strains showed the highest degradation limited to the small colonized area. These results were confirmed by SEM images of the colonization profile on membranes. Mechanical analyses performed on membranes were used as an innovative method to evaluate the thickness and structural integrity of membranes variation following fungal colonization. In conclusion, mechanical analyses of substrate may be used as a procedure for the development of a new onychomycosis diagnosis test in order to develop personalized and strain-specific treatment.

摘要

甲癣是一种由不同病原体引起的真菌感染，这些病原体包括专门定殖于富含角蛋白基质的皮肤癣菌。本研究的目的是调查与[具体菌种]接触的牛膜（用作体外指甲模型）的机械变化。[具体菌种]菌株从趾甲标本中分离得到。实验步骤为：将[具体菌种]菌株接种在含有基本培养基和丰富培养基的培养皿上，然后将牛膜置于培养皿中央。27天后，[具体菌种]显著降低了被定殖膜的厚度，而两株[具体菌种]菌株仅在小面积定殖区域表现出最高程度的降解。膜上定殖情况的扫描电子显微镜图像证实了这些结果。对膜进行的力学分析作为一种创新方法，用于评估真菌定殖后膜的厚度和结构完整性变化。总之，对基质的力学分析可作为开发新型甲癣诊断测试的一种方法，以便制定个性化的、针对特定菌株的治疗方案。

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