Ribeiro-Silva Cárita S, Muniz Elen R, Lima Valesca H, Bernardo Cíntia C, Arruda Walquíria, Castro Rosane N, Gôlo Patrícia S, Angelo Isabele C, Fernandes Éverton K K
Departamento de Biociências e Tecnologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia 74690-900, Brazil.
Faculdade Integradas da América do Sul, Caldas Novas 74692-532, Brazil.
J Fungi (Basel). 2022 Nov 8;8(11):1177. doi: 10.3390/jof8111177.
The chemical composition of tick cuticles acts as a barrier to pathogens and may limit infection by entomopathogenic fungi. This study characterized the cuticular neutral lipids (NL) and hydrocarbons (HCs) of four ixodid ticks that are widely distributed in Brazil. HC extracts were analyzed by gas chromatography-mass spectrometry and used to challenge IP361 and IP146; the effect of cuticular extracts in fungal growth were evaluated by disk diffusion and conidial viability assays. In addition, conidial germination on the tick cuticle was evaluated by scanning electron microscopy, and NL from ticks treated with fungi were assessed by thin layer chromatography. Six HCs were exclusively identified in . Additionally, cuticle extracts from and inhibited the growth of IP146 and reduced conidial germination of IP361 to 70% and 49%, respectively; the same extracts also produced cytotoxic effects, with conidial death above 30% and 60%. Electron micrographs showed a delayed germination of conidia incubated for 48 h or 72 h on and . The lipid profile of treated with fungi was not significantly altered; triacylglycerol was not detected in the cuticle extracts of any other tick species. Finally, and cuticles have lipid components that may limit the development of
蜱虫表皮的化学成分可作为病原体的屏障，并可能限制昆虫病原真菌的感染。本研究对巴西广泛分布的四种硬蜱的表皮中性脂质（NL）和碳氢化合物（HCs）进行了表征。通过气相色谱 - 质谱法分析HC提取物，并用于挑战IP361和IP146；通过纸片扩散法和分生孢子活力测定评估表皮提取物对真菌生长的影响。此外，通过扫描电子显微镜评估蜱虫表皮上的分生孢子萌发情况，并通过薄层色谱法评估用真菌处理的蜱虫的NL。在……中仅鉴定出六种HCs。此外，……和……的表皮提取物抑制了IP146的生长，并分别将IP361的分生孢子萌发率降低至70%和49%；相同的提取物还产生了细胞毒性作用，分生孢子死亡率分别高于30%和60%。电子显微镜照片显示，在……和……上培养48小时或72小时的分生孢子萌发延迟。用真菌处理的……的脂质谱没有显著改变；在任何其他蜱虫种类的表皮提取物中均未检测到三酰甘油。最后，……和……的表皮具有可能限制……发育的脂质成分
