Faculty of Medicine, Health & Life Science, Swansea University, Swansea, UK.
Methods Mol Biol. 2023;2598:29-44. doi: 10.1007/978-1-0716-2839-3_4.
Experimental data suggests that tissue-specific progenitors are present within hyaline articular cartilage with the potential to contribute to growth, maintenance, and repair. In this chapter, we show how colony-forming progenitor-like cells can be isolated from bovine articular cartilage using differential adhesion to fibronectin. Furthermore, we describe the optimal conditions and factors required to differentiate these progenitor cells to produce hyaline articular cartilage.
实验数据表明，透明软骨中存在组织特异性祖细胞，具有促进生长、维持和修复的潜力。在本章中，我们展示了如何使用纤维连接蛋白的差异黏附从牛关节软骨中分离出类集落形成祖细胞样细胞。此外，我们还描述了分化这些祖细胞生成透明关节软骨所需的最佳条件和因素。
