Articular Cartilage Chondroprogenitors: Isolation and Directed Differentiation.

Experimental data suggests that tissue-specific progenitors are present within hyaline articular cartilage with the potential to contribute to growth, maintenance, and repair. In this chapter, we show how colony-forming progenitor-like cells can be isolated from bovine articular cartilage using differential adhesion to fibronectin. Furthermore, we describe the optimal conditions and factors required to differentiate these progenitor cells to produce hyaline articular cartilage.