Department of Mechanical & Industrial Engineering, Montana State University, Bozeman, MT, USA.
Molecular Biosciences Program, Montana State University, Bozeman, MT, USA.
Methods Mol Biol. 2023;2598:141-156. doi: 10.1007/978-1-0716-2839-3_11.
Metabolism has long been recognized as a critical physiological process necessary to maintain homeostasis in all types of cells including the chondrocytes of articular cartilage. Alterations in metabolism in disease and metabolic adaptation to physiological stimuli such as mechanical loading are increasingly recognized as important for understanding musculoskeletal systems such as synovial joints. Metabolomics is an emerging technique that allows quantitative measurement of thousands of small molecule metabolites that serve as both products and reactants to myriad reactions of cellular biochemistry. This protocol describes procedures to perform metabolomic profiling on chondrocytes and other tissues and fluids within the synovial joint.
代谢过程一直被认为是所有类型细胞（包括关节软骨中的软骨细胞）维持内环境稳定的关键生理过程。疾病中的代谢改变以及对生理刺激（如机械加载）的代谢适应，对于理解包括滑膜关节在内的肌肉骨骼系统变得越来越重要。代谢组学是一种新兴技术，可以定量测量数以千计的小分子代谢物，这些代谢物既是细胞生物化学众多反应的产物，也是反应物。本方案描述了在滑膜关节中的软骨细胞和其他组织及体液中进行代谢组学分析的程序。
