Welhaven Hope D, Welfley Avery H, Bothner Brian, Messier Stephen P, Loeser Richard F, June Ronald K
Department of Chemistry & Biochemistry and Molecular Biosciences Program, Montana State University, Bozeman, MT, 59717, USA.
Department of Microbiology and Immunology, Montana State University, Bozeman, MT, USA.
BMC Musculoskelet Disord. 2024 Dec 20;25(1):1057. doi: 10.1186/s12891-024-08166-7.
The Intensive Diet and Exercise for Arthritis (IDEA) trial was a randomized trial conducted to evaluate the effects of diet and exercise on osteoarthritis (OA), the most prevalent form of arthritis. Various risk factors, including obesity and sex, contribute to OA's debilitating nature. While diet and exercise are known to improve OA symptoms, cellular and molecular mechanisms underlying these interventions, as well as effects of participant sex, remain elusive.
Serum was obtained at three timepoints from IDEA participants assigned to groups of diet, exercise, or combined diet and exercise (n = 10 per group). A randomly selected subset of serum samples were extracted and analyzed via liquid chromatography-mass spectrometry combined with metabolomic profiling to unveil mechanisms associated with types of intervention and disease. Extracted serum was pooled and fragmentation patterns were analyzed to identify metabolites that statistically differentially regulated between groups.
Changes in metabolism across male and female IDEA participants after 18-months of diet, exercise, and combined diet and exercise intervention mapped to lipid, amino acid, carbohydrate, vitamin, and matrix metabolism. The diverse metabolic landscape detected across IDEA participants shows that intervention type differentially impacts the serum metabolome of OA individuals. Moreover, dissimilarities in the serum metabolome corresponded with participant sex.
These findings suggest that intensive weight loss among males and females offers potential metabolic benefits for individuals with knee OA. This study provides a deeper understanding of dysregulation occurring during OA development in parallel with various interventions, potentially paving the way for improved interventions, treatments, and quality of life of those impacted by OA.
clinicaltrials.gov Identifier NCT00381290, Registered, 9/25/2006.
关节炎强化饮食与运动（IDEA）试验是一项随机试验，旨在评估饮食和运动对骨关节炎（OA）的影响，OA是最常见的关节炎形式。包括肥胖和性别在内的多种风险因素导致OA具有使人衰弱的特性。虽然已知饮食和运动可改善OA症状，但这些干预措施背后的细胞和分子机制以及参与者性别的影响仍不清楚。
在三个时间点从分配到饮食、运动或饮食与运动相结合组的IDEA参与者中获取血清（每组n = 10）。随机选择一部分血清样本，通过液相色谱 - 质谱联用代谢组学分析来揭示与干预类型和疾病相关的机制。将提取的血清合并并分析碎片模式，以鉴定组间有统计学差异调节的代谢物。
在饮食、运动和饮食与运动相结合的干预18个月后，男性和女性IDEA参与者的代谢变化涉及脂质、氨基酸、碳水化合物、维生素和基质代谢。在IDEA参与者中检测到的多样代谢格局表明，干预类型对OA个体的血清代谢组有不同影响。此外，血清代谢组的差异与参与者性别相对应。
这些发现表明，男性和女性的强化体重减轻对膝OA个体具有潜在的代谢益处。本研究更深入地了解了OA发展过程中与各种干预措施同时发生的失调情况，可能为改善受OA影响者的干预措施、治疗方法和生活质量铺平道路。
clinicaltrials.gov标识符NCT00381290，注册于2006年9月25日。
