Cui Shiwei, Pang Bo, Yan Huifang, Wu Bo, Li Ming, Xing Caihong, Li Juan
Department of Hygienic Inspection, School of Public Health, Jilin University, Changchun 130021, China.
National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Toxics. 2022 Oct 23;10(11):636. doi: 10.3390/toxics10110636.
Urinary benzene metabolites trans, trans-muconic acid (-MA), and S-phenyl mercapturic acid (S-PMA) are often used as biomarkers of internal exposure to benzene. However, there are few reports on using urinary benzene metabolites to estimate airborne benzene concentrations in individuals exposed to benzene. In this study, -MA, and S-PMA were analyzed by UPLC-MS/MS, and a simple pharmacokinetic model was used to calculate the daily intake (DI) of benzene based on the levels of urinary -MA, and S-PMA in occupational individuals. The back-calculated airborne benzene levels (BCABL) were obtained from the DI of benzene. Among the exposed subjects ( = 84), the median BCABL (3.67 mg/m) based on -MA was very close to the median level of measured airborne benzene (3.27 mg/m, = 0.171), and there was no effect of smoking or dietary habits on -MA-based BCABL. In the control subjects ( = 49), the levels of measured airborne benzene were all below the quantitation limit (0.024 mg/m), and the BCABL (0.002-0.25 mg/m) calculated by S-PMA was close to this background level. Our study suggests that the -MA-based BCABL can reflect the actual airborne benzene level in a range of 1.10-86.91 mg/m and that the S-PMA-based BCABL is more reliable for non-professional benzene exposure.
尿中苯代谢物反式、反式粘康酸（t,t - MA）和S - 苯基巯基尿酸（S - PMA）常被用作苯体内暴露的生物标志物。然而，关于利用尿中苯代谢物估算苯暴露个体空气中苯浓度的报道较少。在本研究中，采用超高效液相色谱 - 串联质谱法（UPLC - MS/MS）分析t,t - MA和S - PMA，并基于职业个体尿中t,t - MA和S - PMA水平，使用简单的药代动力学模型计算苯的每日摄入量（DI）。从苯的DI获得反推的空气中苯水平（BCABL）。在暴露组受试者（n = 84）中，基于t,t - MA的BCABL中位数（3.67 mg/m³）与实测空气中苯的中位数水平（3.27 mg/m³，P = 0.171）非常接近，且吸烟或饮食习惯对基于t,t - MA的BCABL无影响。在对照组受试者（n = 49）中，实测空气中苯水平均低于定量限（0.024 mg/m³），通过S - PMA计算的BCABL（0.002 - 0.25 mg/m³）接近此背景水平。我们的研究表明，基于t,t - MA的BCABL在1.10 - 86.91 mg/m³范围内可反映实际空气中苯水平，且基于S - PMA的BCABL对于非职业性苯暴露更可靠。
