Zhao Qiaoya, Tian Ye, Liu Liping, Jiang Yifei, Sun Haitao, Tan Shanjie, Huang Bing
Institute of Poultry Sciences, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Institute of Animal Husbandry and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Vet Sci. 2022 Oct 31;9(11):603. doi: 10.3390/vetsci9110603.
Rabbit astrovirus (RAstV) is a pathogen that causes diarrhea in rabbits, with high infection rate at various stages, which can often cause secondary or mixed infections with other pathogens, bringing great economic losses to the rabbit industry. In this study, 10 samples were collected from cases of rabbits with diarrhea on a rabbit meat farm in the Shandong area of China. The positive sample for astrovirus detected by RT-PCR was inoculated into an RK 13 cell line. A rabbit astrovirus strain named Z317 was successfully isolated, which produced an obvious cytopathic effect 48 h post-inoculation in the RK 13 cell line. The genome structure of this isolate was studied by high-throughput sequencing, showing that the Z317 strain had the highest similarity with the American strain TN/2208/2010, with 92.43% nucleotide homology, belonging to group MRAstV-23. The basic properties of the Z317 capsid (Cap) protein were analyzed, and 10 liner B cell epitopes were screened with the online biosoft Bepipred 2.0 and SVMTriP, including 445-464, 186-205, 655-674, 88-107, 792-811, 45-64, and 257-276 amino acids. This is the first contribution concerning RAstV genomes in China; more studies are needed to understand the diversity and impact of RAstV on rabbit health.
兔星状病毒（RAstV）是一种可导致家兔腹泻的病原体，在各个阶段感染率都很高，常引发与其他病原体的继发或混合感染，给养兔业带来巨大经济损失。在本研究中，从中国山东地区一个肉兔养殖场腹泻病例中采集了10份样本。将经RT-PCR检测为星状病毒阳性的样本接种到RK 13细胞系中。成功分离出一株名为Z317的兔星状病毒毒株，该毒株在RK 13细胞系中接种48小时后产生明显的细胞病变效应。通过高通量测序对该分离株的基因组结构进行研究，结果表明Z317毒株与美国毒株TN/2208/2010相似度最高，核苷酸同源性为92.43%，属于MRAstV-23组。对Z317衣壳（Cap）蛋白的基本特性进行分析，并利用在线生物软件Bepipred 2.0和SVMTriP筛选出10个线性B细胞表位，包括445 - 464、186 - 205、655 - 674、88 - 107、792 - 811、45 - 64和(257 - 276)氨基酸。这是中国有关兔星状病毒基因组的首次研究成果；还需要更多研究来了解兔星状病毒的多样性及其对家兔健康的影响。 （注：原文中257 - 276括号是为了符合英文表述习惯补充，中文翻译时按正常范围表述）
