Winogradsky Institute of Microbiology, Research Centre of Biotechnology RAS, 7/2 Prospekt 60-letiya Oktyabrya, 117312 Moscow, Russia.
Winogradsky Institute of Microbiology, Research Centre of Biotechnology RAS, 7/2 Prospekt 60-letiya Oktyabrya, 117312 Moscow, Russia.
Syst Appl Microbiol. 2022 Nov;45(6):126375. doi: 10.1016/j.syapm.2022.126375. Epub 2022 Oct 21.
A novel facultatively anaerobic moderately thermophilic bacterium, strain B-254, was isolated from a terrestrial hot spring near the town of Goryachinsk in the Baikal lake region (Russian Federation). Motile spherical cells of the strain were present as single cocci, in pairs, or aggregates. The cells had a Gram negative cell wall and reproduced by binary fission. The isolate grew at 30-57 °C (opt. 50-54 °C) and at pH 5.1-8.4 (opt. 6.6-7.1). Strain B-254 was a chemoorganoheterotroph, growing on mono-, di- and polysaccharides (xylan, starch, galactan, galactomannan, xyloglucan, arabinan, curdlan, beta-glucan, locust bean gum, xanthan gum). Sodium chloride or yeast extract were not required for growth. Major cellular fatty acids were iso-C, anteiso-C, and C; major polar lipid was phosphatidylethanolamine. The complete genome of strain B-254 was 5.54 Mb; its GC content was 64 %. According to the results of 16S rRNA gene sequence-based phylogenetic analysis and the conserved proteins sequences-based phylogenomic analysis strain B-254 was on a separate lineage within the order Tepidisphaerales (Phycisphaerae, Planctomycetes). Based on phylogenetic and phylogenomic analyses of Phycisphaerae, whole genome comparisons of Tepidisphaerales as well as distinctive phenotypic features of the strain, it was assigned to a novel genus and species for which the name Fontivita pretiosa gen. nov. sp. nov. is proposed. Strain B-254 = KCTC 82380 = VKM B-3507.
一株新型兼性厌氧中温细菌，菌株 B-254，是从贝加尔湖地区戈里钦斯克镇附近的陆地温泉中分离得到的。该菌株的运动性球形细胞呈单个球菌、成对或聚集存在。细胞具有革兰氏阴性细胞壁，通过二分分裂繁殖。该分离物在 30-57°C（最佳 50-54°C）和 pH5.1-8.4（最佳 6.6-7.1）下生长。菌株 B-254 是一种化能有机异养菌，可在单糖、二糖和多糖（木聚糖、淀粉、半乳糖、半乳甘露聚糖、木葡聚糖、阿拉伯聚糖、纤维二糖、β-葡聚糖、槐豆胶、黄原胶）上生长。生长不需要氯化钠或酵母提取物。主要细胞脂肪酸为 iso-C、anteiso-C 和 C；主要极性脂为磷脂酰乙醇胺。菌株 B-254 的全基因组为 5.54 Mb；GC 含量为 64%。根据 16S rRNA 基因序列基于系统发育分析和保守蛋白序列基于系统发育基因组分析，菌株 B-254 属于厚壁菌门（浮霉菌门、计划菌门）目内的一个单独谱系。根据浮霉菌门的系统发育和系统基因组分析，以及厚壁菌门的全基因组比较以及该菌株的独特表型特征，将其归为一个新属和新种，命名为珍贵 Fontivita pretiosa 属。新种。菌株 B-254=KCTC 82380=VKM B-3507。
