Rackevei Antonia S, Borges Alyssa, Engstler Markus, Dandekar Thomas, Wolf Matthias
Department of Bioinformatics, Biocenter, University of Würzburg, Am Hubland, 97074 Würzburg, Germany.
Department of Cell and Developmental Biology, Biocenter, University of Würzburg, Am Hubland, 97074 Würzburg, Germany.
Biology (Basel). 2022 Nov 4;11(11):1612. doi: 10.3390/biology11111612.
The variable regions (V1-V9) of the 18S rDNA are routinely used in barcoding and phylogenetics. In handling these data for trypanosomes, we have noticed a misunderstanding that has apparently taken a life of its own in the literature over the years. In particular, in recent years, when studying the phylogenetic relationship of trypanosomes, the use of V7/V8 was systematically established. However, considering the current numbering system for all other organisms (including other Euglenozoa), V7/V8 was never used. In Maia da Silva et al. [Parasitology 2004, 129, 549-561], V7/V8 was promoted for the first time for trypanosome phylogenetics, and since then, more than 70 publications have replicated this nomenclature and even discussed the benefits of the use of this region in comparison to V4. However, the primers used to amplify the variable region of trypanosomes have actually amplified V4 (concerning the current 18S rDNA numbering system).
18S rDNA的可变区（V1 - V9）通常用于条形码识别和系统发育研究。在处理锥虫的这些数据时，我们注意到一个误解，多年来它显然在文献中自行其是。特别是近年来，在研究锥虫的系统发育关系时，V7/V8的使用被系统地确立。然而，考虑到所有其他生物（包括其他眼虫纲生物）的当前编号系统，V7/V8从未被使用过。在Maia da Silva等人的研究[《寄生虫学》2004年，第129卷，549 - 561页]中，V7/V8首次被推广用于锥虫系统发育研究，从那时起，超过70篇出版物重复了这个命名法，甚至讨论了与V4相比使用该区域的好处。然而，用于扩增锥虫可变区的引物实际上扩增的是V4（关于当前的18S rDNA编号系统）。
