Wang Pu, Wang Xiaocen, Wang Weirong, Gong Pengtao, Zhang Nan, Zhang Renzhe, Zeng Huan, Sun Qian, Li Wanqing, Li Xin, Cheng Shuqin, Zhang Xu, Huang Xinyi, Gao Chenyang, Zheng Yadong, Li Jianhua, Zhang Xichen
Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Provincial Engineering Research Center for Animal Health Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, China Australia Joint Laboratory for Animal Health Big Data Analytics, College of Animal Science and Technology & College of Veterinary Medicine, Zhejiang A & F University, Hangzhou 311300, China.
Animals (Basel). 2022 Oct 27;12(21):2952. doi: 10.3390/ani12212952.
Neosporosis is caused by (), which mainly infects cattle and goats and severely threatens the animal industry. In this study, the inhibitory effects of polyclonal antiserum anti-NcSRS17, NcSRS2 and NcSRS52 were explored. Cytokines in mice or goat serum were detected after immunization. After infection, the survival of mice was recorded. The pathological changes and parasite loads were observed and detected in tissues. The results showed that anti-NcSRS2, NcSRS17 and NcSRS52 antibodies all inhibit the invasion and proliferation of . The IFN-γ level in the NcSRS17 group was higher than that in the NcSRS2 and NcSRS52 groups, and higher in the NcSRS2-mIL-18 group than in the NcSRS2 group. The survival rates of mice were 16% in the positive control group, 67% in the SRS52 group, 83% in the SRS2 and mIL-18 groups and 100% in the SRS17 and SRS2-mIL-18 groups. Goats immunized with NcSRS17-gIL-18 developed high levels of IL-4, IL-12 and IFN-γ compared with those immunized with NcSRS-17. Parasite loads in the brains of animals in the NcSRS17 and NcSRS17-gIL-18 groups were significantly reduced, and were significantly lower in the NcSRS17-gIL-18 group ( ≤ 0.01). This study indicates that SRS17 may be an antigen candidate for vaccine development against neosporosis, and IL-18 can enhance the immune protective efficiency of antigen candidates.
新孢子虫病由（）引起，主要感染牛和山羊，严重威胁畜牧业。本研究探讨了抗NcSRS17、NcSRS2和NcSRS52多克隆抗血清的抑制作用。免疫后检测小鼠或山羊血清中的细胞因子。感染后，记录小鼠的存活情况。观察并检测组织中的病理变化和寄生虫载量。结果表明，抗NcSRS2、NcSRS17和NcSRS52抗体均能抑制（）的侵袭和增殖。NcSRS17组的IFN-γ水平高于NcSRS2和NcSRS52组，NcSRS2-mIL-18组高于NcSRS2组。阳性对照组小鼠存活率为16%，SRS52组为67%，SRS2和mIL-18组为83%，SRS17和SRS2-mIL-18组为100%。与用NcSRS-17免疫的山羊相比，用NcSRS17-gIL-18免疫的山羊产生了高水平的IL-4、IL-12和IFN-γ。NcSRS17和NcSRS17-gIL-18组动物脑内的寄生虫载量显著降低，且NcSRS17-gIL-18组显著更低（≤0.01）。本研究表明，SRS17可能是抗新孢子虫病疫苗研发的抗原候选物，IL-18可提高抗原候选物的免疫保护效率。
