Liu Jianguo, Qiu Rendong, Liu Ran, Song Pengjie, Lin Pengfei, Chen Huatao, Zhou Dong, Wang Aihua, Jin Yaping
Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.
Animals (Basel). 2022 Oct 28;12(21):2973. doi: 10.3390/ani12212973.
In ruminants, the establishment of pregnancy requires a series of structural and functional changes in the endometrium under the action of hormones, thereby providing an optimal environment for the implantation of the embryo. In this study, we explored the molecular mechanism by which YPEL3 regulates endometrial function during gestation in goats. We found YPEL3 expression was significantly downregulated during early gestation and that YPEL3 overexpression inhibited the expression of , but had no significant effects on the expression of and in goat endometrial epithelial cells (gEECs). In addition, YPEL3 silencing significantly inhibited PGF secretion and the expression of the prostaglandin synthesis-related rate-limiting enzyme-encoding genes and , with no significant effect on the expression of and . Moreover, YPEL3 inhibited the expression of vimentin and β-catenin and pretreatment of gEECs with the β-catenin activator CHIR99021 prevented a YPEL3-induced decrease in vimentin expression. Collectively, our findings confirm that, as a hormone-regulated factor, YPEL3 regulates endometrial function by inhibiting the Wnt/β-catenin signaling pathway and provide new insights for further clarification of the mechanism by which YPEL3 functions during early pregnancy in ruminants.
在反刍动物中，妊娠的建立需要子宫内膜在激素作用下发生一系列结构和功能变化，从而为胚胎着床提供最佳环境。在本研究中，我们探究了YPEL3在山羊妊娠期调节子宫内膜功能的分子机制。我们发现，在妊娠早期YPEL3表达显著下调，并且YPEL3过表达抑制了山羊子宫内膜上皮细胞（gEECs）中[此处原文缺失相关基因名称]的表达，但对[此处原文缺失相关基因名称]和[此处原文缺失相关基因名称]的表达没有显著影响。此外，YPEL3沉默显著抑制了PGF分泌以及前列腺素合成相关限速酶编码基因[此处原文缺失相关基因名称]和[此处原文缺失相关基因名称]的表达，对[此处原文缺失相关基因名称]和[此处原文缺失相关基因名称]的表达没有显著影响。而且，YPEL3抑制波形蛋白和β-连环蛋白的表达，用β-连环蛋白激活剂CHIR99021对gEECs进行预处理可阻止YPEL3诱导的波形蛋白表达降低。总的来说，我们的研究结果证实，作为一种激素调节因子，YPEL3通过抑制Wnt/β-连环蛋白信号通路来调节子宫内膜功能，并为进一步阐明YPEL3在反刍动物早期妊娠期间发挥作用的机制提供了新见解。
