Raza Sayed Haidar Abbas, Khan Rajwali, Cheng Gong, Long Feng, Bing Sun, Easa Ahmed A, Schreurs Nicola M, Pant Sameer D, Zhang Wenzhen, Li Anning, Zan Linsen
College of Animal Science and Technology, Northwest A&F University, Yangling 712100, People's Republic of China; National Beef Cattle Improvement Center, Northwest A&F University, Yangling 712100, People's Republic of China.
College of Animal Science and Technology, Northwest A&F University, Yangling 712100, People's Republic of China; Department of Livestock Management, Breeding and Genetics, The University of Agriculture Peshawar-, Pakistan.
Int J Biol Macromol. 2022 Jan 15;195:198-206. doi: 10.1016/j.ijbiomac.2021.11.202. Epub 2021 Dec 7.
Marbling influences the taste and tenderness of meat and is the main determinant of carcass quality in many countries. This study aims to investigate the influence of KLF6 (Kruppel Like Factor 6) and associated molecular mechanisms on lipid metabolism in bovine adipocytes. In the current study, KLF6 gene expression was down regulated via siRNA (small interfering RNA) in bovine adipocytes in vitro. Subsequently, adipogenic cells were collected from the culture media after 9 days, and subjected to fluorescent imaging and RNA sequencing. After confirming that KLF6 was down regulated in bovine adipocytes by siRNA, differential gene expression analysis was used to characterize the infuence of KLF6 on gene expression profiles in bovine adipocytes. A total of 10,812 genes were characterized as differentially expressed genes (DEGs) of which, 109 were up-regulated and 62 were down-regulated genes. KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis identified that the DEGs were associated with lipid metabolism, carbohydrate metabolism, cell growth and death, cancer, and the signaling pathways for calcium, AMPK (Adenosine Monophosphate-Activated Protein Kinase), PI3K-Akt (Phosphatidylinositol 3-kinase), PPAR (Peroxisome proliferator-activated receptors), MAPK (mitogen-activated protein kinase), cAMP (Cyclic adenosine monophosphate), and Wnt (Wingless-related integration site). Similarly, gene ontology analysis indicated that down-regulation of KLF6 gene significantly up regulated the genes that regulate adipogenesis, differentiation and regulation of adipocytes and homeostasis of bovine adipocytes, specifically regulating the cell-type specific apoptotic action, negative regulation of apoptotic pathways, programmed cell death, and growth. Results indicate that KLF6 has a role in regulating lipid metabolism in bovine adipocytes. These findings provide evidence that may inform further investigations into molecular mechanisms that underlie the role of bovine KLF6 gene in regulating adipogenesis.
大理石花纹影响肉的口感和嫩度，在许多国家是胴体品质的主要决定因素。本研究旨在探讨KLF6（Kruppel样因子6）对牛脂肪细胞脂质代谢的影响及其相关分子机制。在本研究中，通过体外小干扰RNA（siRNA）下调牛脂肪细胞中KLF6基因的表达。随后，在9天后从培养基中收集脂肪生成细胞，并进行荧光成像和RNA测序。在确认siRNA下调了牛脂肪细胞中的KLF6后，采用差异基因表达分析来表征KLF6对牛脂肪细胞基因表达谱的影响。共有10812个基因被鉴定为差异表达基因（DEG），其中109个基因上调，62个基因下调。京都基因与基因组百科全书（KEGG）通路分析表明，这些差异表达基因与脂质代谢、碳水化合物代谢、细胞生长与死亡、癌症以及钙、AMPK（腺苷单磷酸激活蛋白激酶）、PI3K-Akt（磷脂酰肌醇3激酶）、PPAR（过氧化物酶体增殖物激活受体）、MAPK（丝裂原活化蛋白激酶）、cAMP（环磷酸腺苷）和Wnt（无翅相关整合位点）信号通路有关。同样，基因本体分析表明，KLF6基因的下调显著上调了调节脂肪生成、脂肪细胞分化和调节以及牛脂肪细胞稳态的基因，特别是调节细胞类型特异性凋亡作用、凋亡途径的负调节、程序性细胞死亡和生长。结果表明，KLF6在调节牛脂肪细胞脂质代谢中发挥作用。这些发现为进一步研究牛KLF6基因在调节脂肪生成中的作用的分子机制提供了证据。
