Chen Ying-Ju, Er Tze-Kiong
Division of Laboratory Medicine, Asia University Hospital, Asia University, Taichung 413, Taiwan.
Department of Medical Laboratory Science and Biotechnology, Asia University, Taichung 413, Taiwan.
Biomedicines. 2022 Oct 28;10(11):2734. doi: 10.3390/biomedicines10112734.
This study was conducted to evaluate the distribution of respiratory viral pathogens in the emergency department during the coronavirus disease 2019 (COVID-19) pandemic. Between May 2020 and September 2022, patients aged between 0.1 and 98 years arrived at the emergency department of Asia University Hospital, and samples from nasopharyngeal swabs were tested by the FilmArray Respiratory Panel (RP). SARS-CoV-2 positivity was subsequently retested by the cobas Liat system. There were 804 patients for whom the FilmArray RP was tested, and 225 (27.9%) of them had positive results for respiratory viruses. Rhinovirus/enterovirus was the most commonly detected pathogen, with 170 (61.8%) cases, followed by adenovirus with 38 (13.8%), SARS-CoV-2 with 16 (5.8%) cases, and coronavirus 229E, with 16 (5.8%) cases. SARS-CoV-2 PCR results were positive in 16 (5.8%) cases, and there were two coinfections of SARS-CoV-2 with adenovirus and rhinovirus/enterovirus. A total of 43 (5.3%) patients were coinfected; the most coinfection was adenovirus plus rhinovirus/enterovirus, which was detectable in 18 (41.9%) cases. No atypical pathogens were found in this study. Intriguingly, our results showed that there was prefect agreement between the detection of SARS-CoV-2 conducted with the cobas Liat SARS-CoV-2 and influenza A/B nucleic acid test and the FilmArray RP. Therefore, the FilmArray RP assay is a reliable and feasible method for the detection of SARS-CoV-2. In summary, FilmArray RP significantly broadens our capability to detect multiple respiratory infections due to viruses and atypical bacteria. It provides a prompt evaluation of pathogens to enhance patient care and clinical selection strategies in emergency departments during the COVID-19 pandemic.
本研究旨在评估2019冠状病毒病（COVID-19）大流行期间急诊科呼吸道病毒病原体的分布情况。2020年5月至2022年9月期间，年龄在0.1岁至98岁之间的患者前往亚洲大学附属医院急诊科就诊，鼻咽拭子样本通过FilmArray呼吸道检测板（RP）进行检测。随后使用cobas Liat系统对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）阳性结果进行复测。共有804例患者接受了FilmArray RP检测，其中225例（27.9%）呼吸道病毒检测结果呈阳性。鼻病毒/肠道病毒是最常检测到的病原体，有170例（61.8%），其次是腺病毒38例（13.8%），SARS-CoV-2 16例（5.8%），以及冠状病毒229E 16例（5.8%）。16例（5.8%）患者的SARS-CoV-2聚合酶链反应（PCR）结果呈阳性，有2例SARS-CoV-2与腺病毒以及鼻病毒/肠道病毒合并感染。共有43例（5.3%）患者发生合并感染；最常见的合并感染是腺病毒加鼻病毒/肠道病毒，在18例（41.9%）患者中可检测到。本研究未发现非典型病原体。有趣的是，我们的结果显示，使用cobas Liat SARS-CoV-2和甲型/乙型流感核酸检测法检测SARS-CoV-2与FilmArray RP检测结果完全一致。因此，FilmArray RP检测法是一种检测SARS-CoV-2的可靠且可行的方法。总之，FilmArray RP显著拓宽了我们检测多种病毒和非典型细菌引起的呼吸道感染的能力。它能迅速评估病原体，以加强COVID-19大流行期间急诊科的患者护理和临床选择策略。
