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用于蔬菜汁发酵的菌株——质量与健康方面

Strains for Vegetable Juice Fermentation-Quality and Health Aspects.

作者信息

Voaides Catalina, Boiu-Sicuia Oana, Israel-Roming Florentina, Zamfir Medana, Grosu-Tudor Silvia Simona, Angelescu Iulia Roxana, Cornea Calina Petruta

机构信息

Faculty of Biotechnologies, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59 Marasti Blvd., 011464 Bucharest, Romania.

Department of Microbiology, Institute of Biology Bucharest of the Romanian Academy, 296 Splaiul Independenţei, 060031 Bucharest, Romania.

出版信息

Biomedicines. 2022 Nov 9;10(11):2867. doi: 10.3390/biomedicines10112867.

Abstract

Vegetable juices are new carrier variants for beneficial bacteria, representing an alternative to dairy-fermented products, especially for vegan, strict vegetarian, or allergic consumers. The aim of this study was to characterize several Romanian native lactic acid bacteria (LAB) strains to select valuable nutritional and probiotic strains for vegetable juice fermentation. Nineteen LAB strains were analyzed for antibiotic susceptibility (disc-diffusion method), the presence of antibiotic resistance genes, the presence of functional genes. and the production of organic acids by HPLC. Antibiotic resistant strains were observed only with ampicillin (Amp10) and kanamycin (K30), 79% and 32%, respectively, with results partially confirmed by molecular analysis. Multiplex PCR revealed the presence of , , , , , , and genes, related to stress resistance, starch metabolism, and production of vitamins, except for . HPLC analyses were performed on beet roots (SF), tomato (TM), and a mixture of carrots, celery, and beet (MTS) juices. High values of lactic acid were recorded in all cases of LAB fermentation (5034-14,176 µg/mL). The maximum values recorded for acetic acid did not exceed 2.5 mg/mL having a positive influence on the product's taste.

摘要

蔬菜汁是有益细菌的新型载体变体,是乳制品发酵产品的替代品,尤其适用于纯素食者、严格素食者或过敏消费者。本研究的目的是对几种罗马尼亚本土乳酸菌(LAB)菌株进行表征,以选择有价值的营养和益生菌株用于蔬菜汁发酵。通过纸片扩散法分析了19株LAB菌株的抗生素敏感性、抗生素抗性基因的存在情况、功能基因的存在情况,并通过高效液相色谱法分析了有机酸的产生情况。仅在氨苄青霉素(Amp10)和卡那霉素(K30)检测中观察到抗生素抗性菌株,分别为79%和32%,分子分析部分证实了结果。多重PCR揭示了与应激抗性、淀粉代谢和维生素产生相关的 、 、 、 、 、 和 基因的存在,但 除外。对甜菜根(SF)、番茄(TM)以及胡萝卜、芹菜和甜菜混合汁(MTS)进行了高效液相色谱分析。在所有LAB发酵的情况下都记录到了较高的乳酸值(5034-14176 µg/mL)。记录到的乙酸最大值不超过2.5 mg/mL,对产品口感有积极影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b1c/9687318/a715a0247baf/biomedicines-10-02867-g001.jpg

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