Pristavu Mircea-Cosmin, Diguță Filofteia Camelia, Aldea Alexandru Constantin, Badea Florentina, Dragoi Cudalbeanu Mihaela, Ortan Alina, Matei Florentina
Faculty of Biotechnologies, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59 Marasti Blvd., 011464 Bucharest, Romania.
Faculty of Land Reclamation and Environmental Engineering, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59 Mărăsti Blvd., 011464 Bucharest, Romania.
Microorganisms. 2025 Jun 10;13(6):1348. doi: 10.3390/microorganisms13061348.
The rising threat of antimicrobial resistance (AMR) has driven the search for safe and effective alternatives to conventional antibiotics. This study investigated the probiotic potential and postbiotic properties of (one strain), (five strains), and (five strains), identified by 16S rRNA sequencing. Among the strains, demonstrated robust survival under simulated gastrointestinal conditions. Cell surface analyses revealed strong auto-aggregation and hydrophobicity in selected strains, notably MI124 and MI127. Enzymatic profiling revealed potential complex metabolic capabilities across different strains. Safety assessments confirmed the absence of hemolytic and gelatinase activities across all strains. Antibiotic susceptibility testing showed resistance to certain β-lactams, while susceptibility to chloramphenicol and tetracycline varied. All LAB strains demonstrated high freeze-drying survivability, exceeding 78.69%. The antibacterial activity of CFSs was confirmed against 14 Gram-positive and Gram-negative pathogens, with results supporting their potential as antimicrobial agents. The CFSs demonstrated a higher total phenolic content (TPC) and displayed significant antioxidant activity, while the total flavonoid content (TFC) remained consistent across most strains. An FTIR spectral analysis confirmed the presence of key functional groups associated with phenolics, organic acids, and peptides, indicating a complex biochemical profile. Probiotics and their postbiotic derivatives offer promising health benefits, including pathogen inhibition and immune modulation. These findings highlight several LAB strains with promising probiotic traits and postbiotic efficacy, supporting their potential use in functional foods and therapeutic applications.
抗菌耐药性（AMR）日益增长的威胁推动了人们寻找传统抗生素的安全有效替代品。本研究通过16S rRNA测序鉴定了[一种菌株名称]、[五种菌株名称]和[五种菌株名称]的益生菌潜力和后生元特性。在这些菌株中，[具体菌株名称]在模拟胃肠道条件下表现出强大的生存能力。细胞表面分析显示，选定菌株，特别是[具体菌株名称]MI124和[具体菌株名称]MI127，具有很强的自聚集性和疏水性。酶谱分析揭示了不同菌株潜在的复杂代谢能力。安全性评估证实所有菌株均无溶血和明胶酶活性。抗生素敏感性测试表明，某些菌株对某些β-内酰胺类抗生素具有耐药性，而对氯霉素和四环素的敏感性各不相同。所有乳酸菌菌株均表现出较高的冻干存活率，超过78.69%。证实了发酵上清液（CFSs）对14种革兰氏阳性和革兰氏阴性病原体具有抗菌活性，结果支持它们作为抗菌剂的潜力。CFSs表现出较高的总酚含量（TPC）并具有显著的抗氧化活性，而大多数菌株的总黄酮含量（TFC）保持一致。傅里叶变换红外光谱（FTIR）分析证实了与酚类、有机酸和肽相关的关键官能团的存在，表明其具有复杂的生化特征。益生菌及其后生元衍生物具有有益健康的前景，包括抑制病原体和调节免疫。这些发现突出了几种具有良好益生菌特性和后生元功效的乳酸菌菌株，支持它们在功能性食品和治疗应用中的潜在用途。
