Saelee Krittaya, Lulitanond Aroonlug, Sae-Ung Nattaya, Prasongwatana Vitoon, Boonsiri Patcharee, Tavichakorntrakool Ratree
Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
School of Medical Technology, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
Diagnostics (Basel). 2022 Oct 31;12(11):2651. doi: 10.3390/diagnostics12112651.
and are common single- and polymicrobial urinary tract infections which can survive under various oxygen levels, including inside of stone matrices. Therefore, we aimed to investigate and compare the calcium oxalate monohydrate (COM) lithogenic activities including COM crystal growth and aggregation under microaerobic conditions of and isolated from the same stone matrix. The crystal growth was analyzed at the delta crystal area while the crystal aggregation was analyzed as the number of crystal aggregates. The results showed that compared to blank control, , and the co-culture of and were able to significantly promote COM crystal growth under microaerobic conditions. Interestingly, the delta crystal area in the co-culture under microaerobic conditions was larger than that of alone and alone. In addition, only alone and the co-culture were able to significantly increase COM aggregates. This study demonstrated that single- and co-culture of and could promote COM crystal growth and aggregation under microaerobic conditions. The co-culture of and may provide the combination effect on COM crystal interactions. The bacterial surfaces and the important effects on bacteria-crystal interactions should be further evaluated.
[细菌名称1]和[细菌名称2]是常见的单菌和多菌性尿路感染病原体，它们能够在包括结石基质内部在内的各种氧水平下存活。因此，我们旨在研究和比较从同一结石基质中分离出的[细菌名称1]和[细菌名称2]在微需氧条件下的一水草酸钙（COM）成石活性，包括COM晶体生长和聚集情况。在晶体面积增量处分析晶体生长情况，而将晶体聚集分析为晶体聚集体的数量。结果表明，与空白对照相比，[细菌名称1]、[细菌名称2]以及[细菌名称1]和[细菌名称2]的共培养物在微需氧条件下能够显著促进COM晶体生长。有趣的是，微需氧条件下共培养物中的晶体面积增量大于单独培养的[细菌名称1]和单独培养的[细菌名称2]。此外，只有单独培养的[细菌名称2]以及共培养物能够显著增加COM聚集体。本研究表明，[细菌名称1]的单培养以及[细菌名称1]和[细菌名称2]的共培养在微需氧条件下可促进COM晶体生长和聚集。[细菌名称1]和[细菌名称2]的共培养可能对COM晶体相互作用产生联合效应。细菌表面以及对细菌 - 晶体相互作用的重要影响应进一步评估。
