Aix Marseille University Faculté de Médecine t de Pharmacie, IRD, APHM, MEPHI, IHU Méditerranée Infection, 13385 Marseille, France.
IHU Méditerranée Infection, Boulevard Jean Moulin, 13385 Marseille, France.
Cells. 2022 Nov 4;11(21):3495. doi: 10.3390/cells11213495.
In addition to their role in haemostasis, platelets are also involved in the inflammatory and antimicrobial process. Interactions between pathogens and platelets, mediated by receptors can lead to platelet activation, which may be responsible for a granular secretion process or even aggregation, depending on the bacterial species. Granular secretion releases peptides with bactericidal activity as well as aggregating factors. To our knowledge, these interactions have been poorly studied for (). Few studies have characterised the cellular organization of platelet- aggregates. The objective of our study was to investigate the structure of platelet aggregates induced by different strains as well as the ultrastructure of platelet- mixtures using a scanning and transmission electron microscopy (SEM and TEM) approach. Our results show that the appearance of platelet aggregates is mainly dependent on the strain used. SEM images illustrate the platelet activation and aggregation and their colocalisation with bacteria. Some strains induce platelet activation and aggregation, and the bacteria are trapped in the platelet magma. However, some strains do not induce significant platelet activation and are found in close proximity to the platelets. The structure of the strains might explain the results obtained.
除了在止血中的作用外，血小板还参与炎症和抗菌过程。病原体与血小板之间通过受体相互作用可导致血小板激活，这可能导致颗粒分泌过程甚至聚集，具体取决于细菌种类。颗粒分泌释放具有杀菌活性和聚集因子的肽。据我们所知，这些相互作用在（）方面研究甚少。很少有研究描述血小板聚集体的细胞组织。我们研究的目的是使用扫描和透射电子显微镜（SEM 和 TEM）方法研究不同（）菌株诱导的血小板聚集体的结构以及血小板混合物的超微结构。我们的结果表明，血小板聚集体的出现主要取决于所使用的菌株。SEM 图像说明了血小板的激活和聚集及其与细菌的共定位。一些（）菌株诱导血小板的激活和聚集，并且细菌被困在血小板岩浆中。然而，一些菌株不会引起明显的血小板激活，并且与血小板紧密相邻。（）菌株的结构可以解释所获得的结果。
