Jia Kaiying, Wei Min, He Yao, Wang Yujie, Wei Hua, Tao Xueying
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Foods. 2022 Nov 7;11(21):3538. doi: 10.3390/foods11213538.
Exopolysaccharide (EPS) from probiotic WEHI01 was isolated and purified by anion exchange chromatography and gel chromatography, the results of which show that the EPS consists of four fractions, namely I01-1, I01-2, I01-3, and I01-4. As the main purification components, I01-2 and I01-4 were preliminarily characterized for their structure and their immunomodulatory activity was explored. The molecular weight of I01-2 was 2.28 × 10 Da, which consists mainly of galactose, and a few other sugars including glucose, arabinose, mannose, xylose, fucose, and rhamnose, while the I01-4 was composed of galactose only and has a molecular weight of 2.59 × 10 Da. Furthermore, the results of an evaluation of immunomodulatory activity revealed that I01-2 and I01-4 could improve the viability of macrophage cells, improve phagocytosis, boost NO generation, and encourage the release of cytokines including TNF-α and IL-6 in RAW 264.7 macrophages. These results imply that I01-2 and I01-4 could improve macrophage-mediated immune responses and might be useful in the production of functional food and medications.
通过阴离子交换色谱法和凝胶色谱法分离并纯化了来自益生菌WEHI01的胞外多糖（EPS），结果表明该EPS由四个组分组成，即I01-1、I01-2、I01-3和I01-4。作为主要的纯化组分，对I01-2和I01-4的结构进行了初步表征，并探索了它们的免疫调节活性。I01-2的分子量为2.28×10 Da，主要由半乳糖以及包括葡萄糖、阿拉伯糖、甘露糖、木糖、岩藻糖和鼠李糖在内的其他几种糖类组成，而I01-4仅由半乳糖组成，分子量为2.59×10 Da。此外，免疫调节活性评估结果显示，I01-2和I01-4可提高巨噬细胞的活力，增强吞噬作用，促进一氧化氮生成，并促进RAW 264.7巨噬细胞中包括肿瘤坏死因子-α和白细胞介素-6在内的细胞因子释放。这些结果表明，I01-2和I01-4可改善巨噬细胞介导的免疫反应，可能在功能性食品和药物生产中有用。
