Key Laboratory of Animal Genetics Breeding and Reproduction of Ministry of Agriculture and Rural Affairs of China, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518124, China.
Genes (Basel). 2022 Oct 28;13(11):1971. doi: 10.3390/genes13111971.
encodes the type I collagen α1 chain, which shows the highest abundance among members of the collagen family and is widely expressed in different mammalian cells and tissues. However, its molecular characteristics are not completely elucidated. In this study, the molecular profiles of and characteristics of the COL1A1 protein were investigated using a promoter activity assay and multiple bioinformatics tools. The results showed that the 5' flanking region of porcine contained two CpG islands, five core promoter sequences, and twenty-six transcription factor-binding sites. In the luciferase assay, the upstream 294 bp region of the initiation codon of showed the highest activity, confirming that this section is the core region of the porcine promoter. Bioinformatic analysis revealed that COL1A1 is a negatively charged, hydrophilic secreted protein. It does not contain a transmembrane domain and is highly conserved in humans, mice, sheep, and pigs. Protein interaction analysis demonstrated that the interaction coefficient of COL1A1 with COL1A2, COL3A1, ITGB1, and ITGA2 was greater than 0.9, suggesting that this protein plays a crucial role in collagen structure formation and cell adhesion. These results provide a theoretical basis for further investigation of the functions of porcine .
编码 I 型胶原 α1 链，该蛋白在胶原家族成员中丰度最高，广泛表达于不同的哺乳动物细胞和组织中。然而，其分子特征尚未完全阐明。本研究采用启动子活性测定和多种生物信息学工具，研究了 和 COL1A1 蛋白的分子特征。结果表明，猪 的 5'侧翼区含有两个 CpG 岛、五个核心启动子序列和二十六个转录因子结合位点。在荧光素酶检测中，起始密码子上游 294bp 区域显示出最高的活性，证实该区域是猪 启动子的核心区域。生物信息学分析表明，COL1A1 是一种带负电荷的亲水分泌型蛋白，不含有跨膜结构域，在人类、小鼠、绵羊和猪中高度保守。蛋白相互作用分析表明，COL1A1 与 COL1A2、COL3A1、ITGB1 和 ITGA2 的相互作用系数大于 0.9，提示该蛋白在胶原结构形成和细胞黏附中发挥重要作用。这些结果为进一步研究猪 的功能提供了理论基础。
