Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, Kasztanowa 3, 41-200 Sosnowiec, Poland.
"Biochefa" Pharmaceutical Research and Production Plant, 3, 41-200 Sosnowiec, Poland.
Int J Mol Sci. 2022 Oct 27;23(21):13031. doi: 10.3390/ijms232113031.
Renal ischemia in the peri-transplant period causes a number of changes that adversely affect the initiation of normal vital functions in grafts after transplantation. To minimise the extent of ischemic damage, organs are stored in preservation fluid. The components of the fluid are supposed to ensure stabilisation of the cell cytoskeleton, protect against oxygen free radicals, reduce cell swelling, and ensure endothelial cell integrity. The aim of this study was to analyse the protective effect of Cu, as a component of Biolasol preservative fluid, in the prevention of nephron damage occurring during the graft storage period. Analyses of the effectiveness of copper in the presence of prolactin added to Biolasol fluid were also carried out. Forty isolated pig slaughter kidneys were used in the study, avoiding the use of laboratory animals. The kidneys were stored using simple hypothermia. After 2 h and 48 h of graft storage, selected biochemical indicators of renal function were determined in the collected perfusates. The addition of Cu at a dose of 1 µg/L to the composition of Biolasol fluid was found to affect the generation of ischemic damage in the isolated pig kidney. The intensity of the occurrence of these processes is exacerbated by the presence of prolactin at a dose of 0.1 µg/L.
移植期的肾缺血会引起一系列变化，这些变化会对移植后移植物正常生命功能的启动产生不利影响。为了最大限度地减少缺血损伤的程度，器官被储存在保存液中。该液体的成分应确保细胞细胞骨架的稳定，防止氧自由基，减少细胞肿胀，并确保内皮细胞的完整性。本研究的目的是分析铜作为 Biolasol 保存液成分的保护作用，以预防移植物储存期间发生的肾单位损伤。还分析了添加催乳素的铜在 Biolasol 液体中的有效性。本研究使用 40 个分离的猪屠宰肾脏，避免使用实验动物。肾脏通过简单的低温保存。在移植物储存 2 小时和 48 小时后，在收集的灌流液中测定了所选肾功能的生化指标。在 Biolasol 液体的组成中添加 1 µg/L 的 Cu 被发现会影响分离猪肾的缺血损伤的发生。催乳素剂量为 0.1 µg/L 时，这些过程的发生强度加剧。
Zotero 插件
