Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai 980-8572, Japan.
Faulty of Agriculture, Yamagata University, Tsuruoka 997-8555, Japan.
Int J Mol Sci. 2022 Oct 31;23(21):13288. doi: 10.3390/ijms232113288.
Light quality affects plant growth and the functional component accumulation of fruits. However, there is little knowledge of the effects of light quality based on multiomics profiles. This study combined transcriptomic, ionomic, and metabolomic analyses to elucidate the effects of light quality on metabolism and gene expression in tomato fruit. Micro-Tom plants were grown under blue or red light-emitting diode light for 16 h daily after anthesis. White fluorescent light was used as a reference. The metabolite and element concentrations and the expression of genes markedly changed in response to blue and red light. Based on the metabolomic analysis, amino acid metabolism and secondary metabolite biosynthesis were active in blue light treatment. According to transcriptomic analysis, differentially expressed genes in blue and red light treatments were enriched in the pathways of secondary metabolite biosynthesis, carbon fixation, and glycine, serine, and threonine metabolism, supporting the results of the metabolomic analysis. Ionomic analysis indicated that the element levels in fruits were more susceptible to changes in light quality than in leaves. The concentration of some ions containing Fe in fruits increased under red light compared to under blue light. The altered expression level of genes encoding metal ion-binding proteins, metal tolerance proteins, and metal transporters in response to blue and red light in the transcriptomic analysis contributes to changes in the ionomic profiles of tomato fruit.
光照质量会影响植物生长和果实功能成分的积累。然而，基于多组学分析的光照质量对果实代谢和基因表达的影响的相关知识还很少。本研究结合转录组学、离子组学和代谢组学分析，阐明了光照质量对番茄果实代谢和基因表达的影响。在开花后，每天用蓝色或红色发光二极管光照射 Micro-Tom 植物 16 小时，白色荧光灯作为对照。代谢物和元素浓度以及基因表达对蓝光和红光的响应明显改变。基于代谢组学分析，在蓝光处理中，氨基酸代谢和次生代谢物生物合成活跃。根据转录组学分析，蓝光和红光处理中差异表达的基因在次生代谢物生物合成、碳固定以及甘氨酸、丝氨酸和苏氨酸代谢途径中富集，支持了代谢组学分析的结果。离子组学分析表明，果实中的元素水平比叶片对光照质量的变化更敏感。与蓝光相比，红光下果实中一些含 Fe 的离子浓度增加。转录组学分析中，对蓝光和红光响应的基因编码金属离子结合蛋白、金属耐受蛋白和金属转运蛋白的表达水平发生改变，导致番茄果实离子组学图谱发生变化。
