Pereira Ana C C, Aguiar Alana P S, Araujo Leticia M P, Dantas Larissa O, Mayer Marcia P A, Karygianni Lamprini, Thurnheer Thomas, Pinheiro Ericka T
Department of Dentistry, School of Dentistry, University of São Paulo, São Paulo 05508-000, Brazil.
Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, São Paulo 05508-000, Brazil.
Life (Basel). 2022 Oct 24;12(11):1686. doi: 10.3390/life12111686.
The antimicrobial peptide LL-37 and D-amino acids (D-AAs) have been proposed as antibiofilm agents. Therefore, this study aimed to test the antimicrobial effect of antibiofilm agents associated with antibiotics used in regenerative endodontic procedures (the triple antibiotic paste—TAP: ciprofloxacin + metronidazole + minocycline). An endodontic-like biofilm model grown on bovine dentin discs was used in this study. After 21-day growth, the biofilms were treated with 1 mg/mL TAP, 10 μM LL-37, an association of LL-37 + TAP, 40 mM D-AAs solution, an association of D-AAs + TAP, and phosphate-buffered saline (negative control). Colony forming unit (CFU) data were analyzed by two-way ANOVA and Tukey’s multiple comparison test (p < 0.05). LL-37 + TAP showed the best antibacterial activity (7-log10 CFU/mL ± 0.5), reaching a 1 log reduction of cells in relation to the negative control (8-log10 CFU/mL ± 0.7) (p < 0.05). In turn, no significant reduction in bacterial cells was observed with TAP, LL-37, D-AAs, and D-AAs + TAP compared to the negative control. In conclusion, the combination of antibiotics and LL-37 peptide showed mild antibacterial activity, while the combination of antibiotics and D-AAs showed no activity against complex biofilms.
抗菌肽LL-37和D-氨基酸（D-AAs）已被提议作为抗生物膜剂。因此，本研究旨在测试与再生牙髓治疗中使用的抗生素相关的抗生物膜剂的抗菌效果（三联抗生素糊剂-TAP：环丙沙星+甲硝唑+米诺环素）。本研究使用了在牛牙本质盘上生长的类牙髓生物膜模型。生长21天后，用1mg/mL TAP、10μM LL-37、LL-37+TAP组合、40mM D-AAs溶液、D-AAs+TAP组合和磷酸盐缓冲盐水（阴性对照）处理生物膜。通过双向方差分析和Tukey多重比较检验（p<0.05）分析菌落形成单位（CFU）数据。LL-37+TAP显示出最佳的抗菌活性（7-log10 CFU/mL±0.5），与阴性对照（8-log10 CFU/mL±0.7）相比，细胞减少了1个对数（p<0.05）。相比之下，与阴性对照相比，TAP、LL-37、D-AAs和D-AAs+TAP未观察到细菌细胞的显著减少。总之，抗生素与LL-37肽的组合显示出轻度抗菌活性，而抗生素与D-AAs的组合对复杂生物膜无活性作用。
