Movahedi Ali, Wei Hui, Alhassan Abdul Razak, Dzinyela Raphael, Wang Pu, Sun Weibo, Zhuge Qiang, Xu Chen
College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China.
Key Laboratory of Landscape Plant Genetics and Breeding, School of Life Sciences, Nantong University, Nantong 226019, China.
Life (Basel). 2022 Nov 9;12(11):1830. doi: 10.3390/life12111830.
is a genus of globally significant plantation trees used widely in industrial and agricultural production. Poplars are easily damaged by and , resulting in decreasing quality. toxin-encoded by the gene has been widely adopted in poplar breeding because of its strong insect resistance. There is still no comprehensive and sufficient information about the effects of -modified (CM) poplars on the ecological environment. Here, we sampled the rhizosphere soils of field-grown CM and non-transgenic (NT) poplars and applied 16S rRNA and internal transcribed spacer amplicon Illumina MiSeq sequencing to determine the bacterial community associated with the CM and NT poplars. Based on the high-throughput sequencing of samples, we found that the predominant taxa included (about 40% of the total bacteria), (about 20% of the total bacteria), and (about 20% of the total bacteria) collected from the natural rhizosphere of NT and CM poplars. In addition, studies on the microbial diversity of poplar showed that expression has no significant influence on rhizosphere soil alkaline nitrogen, but significantly affects soil phosphorus, soil microbial biomass nitrogen, and carbon. The results exhibited a similar bacterial community structure between CM varieties affected by the expression of and non-transgenic poplars. In addition, expression revealed no significant influence on the composition of rhizosphere microbiomes. These results broadly reflect the effect of the toxin-encoded by on the ecology and environment and provide a clear path for researchers to continue research in this field in the future.
是一类在全球具有重要意义的人工林树木，广泛应用于工农业生产。杨树容易受到[具体病虫害名称1]和[具体病虫害名称2]的侵害，导致质量下降。由[具体基因名称]基因编码的[具体毒素名称]毒素因其强大的抗虫性已被广泛应用于杨树育种。关于[具体毒素名称]基因修饰（CM）杨树对生态环境的影响，目前仍没有全面且充分的信息。在这里，我们采集了田间种植的CM杨树和非转基因（NT）杨树的根际土壤，并应用16S rRNA和内转录间隔区扩增子Illumina MiSeq测序来确定与CM杨树和NT杨树相关的细菌群落。基于样本的高通量测序，我们发现从NT杨树和CM杨树的天然根际采集的优势类群包括[具体细菌类群名称1]（约占细菌总数的40%）、[具体细菌类群名称2]（约占细菌总数的20%）和[具体细菌类群名称3]（约占细菌总数的20%）。此外，对杨树微生物多样性的研究表明，[具体毒素名称]的表达对根际土壤碱解氮没有显著影响，但对土壤磷、土壤微生物生物量氮和碳有显著影响。结果显示，受[具体毒素名称]表达影响的CM品种与非转基因杨树之间的细菌群落结构相似。此外，[具体毒素名称]的表达对根际微生物群落的组成没有显著影响。这些结果广泛反映了由[具体基因名称]编码的[具体毒素名称]毒素对生态环境的影响，并为研究人员未来在该领域继续开展研究提供了明确的方向。
