Takeshita Junpei, Aoki Shiho, Wada Risei, Osawa Ayako, Sawai Jun
Department of Nutrition and Life Science, Faculty of Health and Medical Sciences, Kanagawa Institute of Technology, 1030 Shimo-Ogino, Atsugi 243-0292, Kanagawa, Japan.
Faculty of Applied Bioscience, Kanagawa Institute of Technology, 1030 Shimo-Ogino, Atsugi 243-0292, Kanagawa, Japan.
Membranes (Basel). 2022 Oct 27;12(11):1049. doi: 10.3390/membranes12111049.
Silicone (polydimethylsiloxane) materials are widely used in various applications. Due to microbe adherence and biofilm formation at the surface of silicone materials, silicone materials must possess antibacterial properties. To achieve this, we prepared copper (Cu)−silicone composite membranes using a simple two-step process of immersion in iodine and copper sulfate solutions. Subsequent scanning electron microscopy revealed Cu nanoparticles (CuNPs) of 10 to 200 nanometers in diameter on the silicone membrane surface, which were identified as copper iodide using energy-dispersive X-ray spectroscopy. The mechanical strength of the material did not change significantly as a result of the two-step immersion treatment and the Cu/silicone membrane showed excellent antibacterial efficacy against Escherichia coli and Staphylococcus aureus, maintaining R > 2 even after a physical impact such as stomacher treatment. Additionally, the Cu ions eluted from the Cu/silicone membrane remained at very low concentrations, suggesting firm immobilization of CuNPs on the silicone membrane. This proposed antimicrobial treatment method does not require special equipment, can be performed at room temperature, and has the potential for use on silicone materials other than membranes.
硅酮（聚二甲基硅氧烷）材料广泛应用于各种领域。由于硅酮材料表面存在微生物附着和生物膜形成的问题，硅酮材料必须具备抗菌性能。为实现这一目标，我们采用简单的两步法，即将材料先后浸入碘溶液和硫酸铜溶液中，制备出铜（Cu）-硅酮复合膜。随后的扫描电子显微镜观察显示，硅酮膜表面存在直径为10至200纳米的铜纳米颗粒（CuNP），通过能量色散X射线光谱分析确定其为碘化铜。经过两步浸泡处理后，材料的机械强度没有显著变化，且铜/硅酮膜对大肠杆菌和金黄色葡萄球菌表现出优异的抗菌效果，即使在经过诸如均质器处理等物理冲击后，其抗菌率仍保持R>2。此外，从铜/硅酮膜上洗脱的铜离子浓度极低，这表明铜纳米颗粒牢固地固定在硅酮膜上。这种提出的抗菌处理方法不需要特殊设备，可在室温下进行，并且有可能应用于除膜以外的其他硅酮材料。
