Department of Sciences, University of Basilicata, Via dell'Ateneo Lucano 10, 85100 Potenza, Italy.
Gnosis Bioresearch by Lesaffre, 75015 Pisticci, Italy.
Molecules. 2022 Nov 2;27(21):7468. doi: 10.3390/molecules27217468.
L-Dopa (LD), a substance used medically in the treatment of Parkinson's disease, is found in several natural products, such as L., also known as broad beans. Due to its low chemical stability, LD analysis in plant matrices requires an appropriate optimization of the chosen analytical method to obtain reliable results. This work proposes an HPLC-UV method, validated according to EURACHEM guidelines as regards linearity, limits of detection and quantification, precision, accuracy, and matrix effect. The LD extraction was studied by evaluating its aqueous stability over 3 months. The best chromatographic conditions were found by systematically testing several C stationary phases and acidic mobile phases. In addition, the assessment of the best storage treatment of L. broad beans able to preserve a high LD content was performed. The best LD determination conditions include sun-drying storage, extraction in HCl 0.1 M, chromatographic separation with a Discovery C column, 250 × 4.6 mm, 5 µm particle size, and 99% formic acid 0.2% / and 1% methanol as the mobile phase. The optimized method proposed here overcomes the problems linked to LD stability and separation, thus contributing to the improvement of its analytical determination.
L-多巴（LD）是一种用于治疗帕金森病的医学物质，存在于几种天然产物中，例如 L.，也称为蚕豆。由于其化学稳定性低，因此需要对所选分析方法进行适当的优化，才能对植物基质中的 LD 进行可靠的分析。本工作提出了一种 HPLC-UV 方法，根据 EURACHEM 指南对其线性、检测限和定量限、精密度、准确度和基质效应进行了验证。通过评估 LD 在 3 个月内的水稳定性来研究其提取情况。通过系统地测试几种 C 固定相和酸性流动相，找到了最佳的色谱条件。此外，还对能够保持高 LD 含量的 L. 蚕豆的最佳储存处理进行了评估。最佳 LD 测定条件包括晒干储存、0.1 M HCl 提取、Discovery C 柱色谱分离、250×4.6mm、5µm 粒径和 99%甲酸 0.2%/和 1%甲醇作为流动相。本研究提出的优化方法克服了 LD 稳定性和分离方面的问题，有助于提高其分析测定的准确性。
