• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

含封装抗菌和抗氧化物质的新型伤口敷料的制备及生物学测试

The Preparation and Biological Testing of Novel Wound Dressings with an Encapsulated Antibacterial and Antioxidant Substance.

作者信息

Braťka Petr, Fenclová Taťána, Hlinková Jana, Uherková Lenka, Šebová Eva, Hefka Blahnová Veronika, Hedvičáková Věra, Žižková Radmila, Litvinec Andrej, Trč Tomáš, Rosina Jozef, Filová Eva

机构信息

Faculty of Biomedical Engineering, Czech Technical University, Náměstí Sítná 3105, 27201 Kladno, Czech Republic.

Grade Medical s.r.o., Náměstí Sítná 3105, 27201 Kladno, Czech Republic.

出版信息

Nanomaterials (Basel). 2022 Oct 29;12(21):3824. doi: 10.3390/nano12213824.

DOI:10.3390/nano12213824
PMID:36364600
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9656126/
Abstract

Chronic wounds represent a significant socio-economic problem, and the improvement of their healing is therefore an essential issue. This paper describes the preparation and biological properties of a novel functionalized nanofiber wound dressing consisting of a polycaprolactone nanofiber carrier modified by a drug delivery system, based on the lipid particles formed by 1-tetradecanol and encapsulated gentamicin and tocopherol acetate. The cytotoxicity of extracts was tested using a metabolic activity assay, and the antibacterial properties of the extracts were tested in vitro on the bacterial strains and . The effect of the wound dressing on chronic wound healing was subsequently tested using a mouse model. Fourteen days after surgery, the groups treated by the examined wound cover showed a lower granulation, reepithelization, and inflammation score compared to both the uninfected groups, a lower dermis organization compared to the control, a higher scar thickness compared to the other groups, and a higher thickness of hypodermis and bacteria score compared to both the uninfected groups. This work demonstrates the basic parameters of the safety (biocompatibility) and performance (effect on healing) of the dressing as a medical device and indicates the feasibility of the concept of its preparation in outpatient conditions using a suitable functionalization device.

摘要

慢性伤口是一个重大的社会经济问题，因此改善其愈合情况是一个至关重要的问题。本文描述了一种新型功能化纳米纤维伤口敷料的制备及其生物学特性，该敷料由聚己内酯纳米纤维载体组成，该载体通过药物递送系统进行了改性，该药物递送系统基于由1-十四醇形成的脂质颗粒，并包裹了庆大霉素和醋酸生育酚。使用代谢活性测定法测试提取物的细胞毒性，并在体外对细菌菌株 和 测试提取物的抗菌性能。随后使用小鼠模型测试伤口敷料对慢性伤口愈合的影响。手术后14天，与未感染组相比，接受检查的伤口覆盖物治疗的组的肉芽组织、再上皮化和炎症评分较低，与对照组相比真皮组织较低，与其他组相比瘢痕厚度较高，与未感染组相比皮下组织厚度和细菌评分较高。这项工作展示了该敷料作为一种医疗器械的安全性（生物相容性）和性能（对愈合的影响）的基本参数，并表明在门诊条件下使用合适的功能化设备制备该敷料概念的可行性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/cb105402eaeb/nanomaterials-12-03824-g015.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/d779f7efc078/nanomaterials-12-03824-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/9a835140affc/nanomaterials-12-03824-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/310b8ecd359b/nanomaterials-12-03824-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/7f7500aed0f4/nanomaterials-12-03824-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/45d42b0ab9b6/nanomaterials-12-03824-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/a782069ca9c8/nanomaterials-12-03824-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/3df364d8e616/nanomaterials-12-03824-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/2443dd01ee2a/nanomaterials-12-03824-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/759dfa1129e3/nanomaterials-12-03824-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/482e2efa9dae/nanomaterials-12-03824-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/7b738ced0cdc/nanomaterials-12-03824-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/c0bf9c2309fd/nanomaterials-12-03824-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/f81e963b6d46/nanomaterials-12-03824-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/9236a686122a/nanomaterials-12-03824-g014.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/cb105402eaeb/nanomaterials-12-03824-g015.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/d779f7efc078/nanomaterials-12-03824-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/9a835140affc/nanomaterials-12-03824-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/310b8ecd359b/nanomaterials-12-03824-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/7f7500aed0f4/nanomaterials-12-03824-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/45d42b0ab9b6/nanomaterials-12-03824-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/a782069ca9c8/nanomaterials-12-03824-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/3df364d8e616/nanomaterials-12-03824-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/2443dd01ee2a/nanomaterials-12-03824-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/759dfa1129e3/nanomaterials-12-03824-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/482e2efa9dae/nanomaterials-12-03824-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/7b738ced0cdc/nanomaterials-12-03824-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/c0bf9c2309fd/nanomaterials-12-03824-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/f81e963b6d46/nanomaterials-12-03824-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/9236a686122a/nanomaterials-12-03824-g014.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2385/9656126/cb105402eaeb/nanomaterials-12-03824-g015.jpg

相似文献

1
The Preparation and Biological Testing of Novel Wound Dressings with an Encapsulated Antibacterial and Antioxidant Substance.含封装抗菌和抗氧化物质的新型伤口敷料的制备及生物学测试
Nanomaterials (Basel). 2022 Oct 29;12(21):3824. doi: 10.3390/nano12213824.
2
Phloretin@cyclodextrin/natural silk protein/polycaprolactone nanofiber wound dressing with antioxidant and antibacterial activities promotes diabetic wound healing.具有抗氧化和抗菌活性的根皮苷@环糊精/天然丝蛋白/聚己内酯纳米纤维伤口敷料促进糖尿病伤口愈合。
Int J Biol Macromol. 2024 Nov;280(Pt 2):135724. doi: 10.1016/j.ijbiomac.2024.135724. Epub 2024 Sep 16.
3
[Effects of reactive oxygen species-responsive antibacterial microneedles on the full-thickness skin defect wounds with bacterial colonization in diabetic mice].活性氧响应性抗菌微针治疗糖尿病小鼠全层皮肤缺损伴细菌定植伤口的效果
Zhonghua Shao Shang Za Zhi. 2021 Nov 20;37(11):1024-1035. doi: 10.3760/cma.j.cn501120-20210831-00299.
4
Honey/Chitosan Nanofiber Wound Dressing Enriched with Allium sativum and Cleome droserifolia: Enhanced Antimicrobial and Wound Healing Activity.富含大蒜和白花菜的蜂蜜/壳聚糖纳米纤维伤口敷料：增强抗菌和伤口愈合活性。
ACS Appl Mater Interfaces. 2016 Mar;8(10):6379-90. doi: 10.1021/acsami.6b00739. Epub 2016 Mar 7.
5
Mussel-inspired adhesive antioxidant antibacterial hemostatic composite hydrogel wound dressing via photo-polymerization for infected skin wound healing.通过光聚合制备的贻贝启发式粘性抗氧化抗菌止血复合水凝胶伤口敷料用于感染性皮肤伤口愈合
Bioact Mater. 2021 Jun 23;8:341-354. doi: 10.1016/j.bioactmat.2021.06.014. eCollection 2022 Feb.
6
Antimicrobial effects of bacterial binding to a dialkylcarbamoyl chloride-coated wound dressing: an in vitro study.一种二烷基碳酰氯涂层伤口敷贴材料对细菌结合的抗菌作用：体外研究。
J Wound Care. 2022 Jul 2;31(7):560-570. doi: 10.12968/jowc.2022.31.7.560.
7
Biocompatible curdlan-based biomaterials loaded with gentamicin and Zn-doped nano-hydroxyapatite as promising dressing materials for the treatment of infected wounds and prevention of surgical site infections.载有庆大霉素和掺锌纳米羟基磷灰石的生物相容性凝乳多糖基生物材料，可用作治疗感染性伤口和预防手术部位感染的有前途的敷料材料。
Biomater Adv. 2022 Aug;139:213006. doi: 10.1016/j.bioadv.2022.213006. Epub 2022 Jun 28.
8
[Antiseptic effect of compound lysostaphin disinfectant and its preventive effect on infection of artificial dermis after graft on full-thickness skin defect wound in rats].[复方溶葡萄球菌酶消毒剂的抗菌效果及其对大鼠全层皮肤缺损创面植皮后人工真皮感染的预防作用]
Zhonghua Shao Shang Za Zhi. 2018 Apr 20;34(4):225-232. doi: 10.3760/cma.j.issn.1009-2587.2018.04.007.
9
Antibacterial properties of functionalized silk fibroin and sericin membranes for wound healing applications in oral and maxillofacial surgery.用于口腔颌面外科学中伤口愈合应用的功能化丝素蛋白和丝胶膜的抗菌性能。
Biomater Adv. 2022 Apr;135:212740. doi: 10.1016/j.bioadv.2022.212740. Epub 2022 Mar 17.
10
Novel lipophosphonoxin-loaded polycaprolactone electrospun nanofiber dressing reduces Staphylococcus aureus induced wound infection in mice.新型载脂磷霉素聚己内酯电纺纳米纤维敷料减轻金黄色葡萄球菌诱导的小鼠创面感染。
Sci Rep. 2021 Sep 3;11(1):17688. doi: 10.1038/s41598-021-96980-7.

本文引用的文献

1
Synergistic effects of quercetin and vitamin E on egg production, egg quality, and immunity in aging breeder hens.槲皮素和维生素 E 对老龄种母鸡产蛋性能、蛋品质和免疫功能的协同作用。
Poult Sci. 2021 Dec;100(12):101481. doi: 10.1016/j.psj.2021.101481. Epub 2021 Sep 16.
2
α-Tocopherol-loaded reactive oxygen species-scavenging ferrocene nanocapsules with high antioxidant efficacy for wound healing.载有α-生育酚的活性氧清除二茂铁纳米胶囊，具有高效抗氧化功效，可用于伤口愈合。
Int J Pharm. 2021 Mar 1;596:120205. doi: 10.1016/j.ijpharm.2021.120205. Epub 2021 Jan 22.
3
Vitamin E in Atopic Dermatitis: From Preclinical to Clinical Studies.
维生素 E 在特应性皮炎中的作用：从临床前研究到临床研究。
Dermatology. 2021;237(4):553-564. doi: 10.1159/000510653. Epub 2020 Oct 16.
4
Advances and Impact of Antioxidant Hydrogel in Chronic Wound Healing.抗氧化水凝胶在慢性伤口愈合中的进展与影响
Adv Healthc Mater. 2020 Mar;9(5):e1901502. doi: 10.1002/adhm.201901502. Epub 2020 Jan 24.
5
New Evidence and Insights on Dalbavancin and Wound Healing in a Mouse Model of Skin Infection.新证据和新见解：达巴万星在皮肤感染小鼠模型中的疗效和伤口愈合情况。
Antimicrob Agents Chemother. 2020 Mar 24;64(4). doi: 10.1128/AAC.02062-19.
6
Vitamin E for Prevention of Biofilm-caused Healthcare-associated Infections.维生素E预防生物膜引起的医疗相关感染
Open Med (Wars). 2019 Dec 26;15:14-21. doi: 10.1515/med-2020-0004. eCollection 2018.
7
Antimicrobial Silver Nanoparticles for Wound Healing Application: Progress and Future Trends.用于伤口愈合的抗菌银纳米颗粒：进展与未来趋势
Materials (Basel). 2019 Aug 9;12(16):2540. doi: 10.3390/ma12162540.
8
Poly-ε-caprolactone and polyvinyl alcohol electrospun wound dressings: adhesion properties and wound management of skin defects in rabbits.聚己内酯和聚乙烯醇电纺伤口敷料：对兔皮肤缺损的黏附性能和伤口管理。
Regen Med. 2019 May;14(5):423-445. doi: 10.2217/rme-2018-0072. Epub 2019 Jun 10.
9
Novel zein-based multilayer wound dressing membranes with controlled release of gentamicin.新型玉米醇溶蛋白基多层创伤敷料膜，具有庆大霉素控释功能。
J Biomed Mater Res B Appl Biomater. 2019 Aug;107(6):2057-2070. doi: 10.1002/jbm.b.34298. Epub 2018 Dec 21.
10
Vitamin E Increases Antimicrobial Sensitivity by Inhibiting Bacterial Lipocalin Antibiotic Binding.维生素 E 通过抑制细菌脂磷壁酸抗生素结合来增加抗菌敏感性。
mSphere. 2018 Dec 12;3(6):e00564-18. doi: 10.1128/mSphere.00564-18.