Bezerra Roberto Alves, Lima Brendo Andrade, Alvares Felipe Boniedj Ventura, Rossi Gabriel Augusto Marques, Braga Fabio Ribeiro, de Melo Renata Pimentel Bandeira, Mota Rinaldo Aparecido, Vilela Vinícius Longo Ribeiro, Feitosa Thais Ferreira
Laboratory of Immunology and Infectious Diseases, Department of Veterinary Medicine, Instituto Federal da Paraíba (IFPB), Sousa 58807-630, PA, Brazil.
Post-Graduating Program of Science and Animal Health, Universidade Federal de Campina Grande (UFCG), Patos 58708-110, PA, Brazil.
Pathogens. 2022 Oct 29;11(11):1263. doi: 10.3390/pathogens11111263.
The aim was to detect correlations of IgG antibodies against in serum and colostrum samples from ewes, through the IFAT, and to evaluate the presence of this immunoglobulin in the serum of newborn lambs after colostrum ingestion. Blood samples from 162 ewes that did not show any disease in the general physical examination and from their newborn lambs, not more than five days postpartum, along with 162 colostrum samples and 182 blood samples from the neonates, were analyzed. In total, 27.8% (45/162) of the mothers were positive for anti- IgG, among which antibodies were detected in the colostrum in 46.7% (21/45). All the ewes with positive colostrum had reactive offspring. The kappa agreement for the correlation between the serological tests on the ewes and the colostrum results was 0.558. This correlation increased as the antibody titers of the mothers increased, and reached 1.000 from the titer of 1:400 from the mothers. Comparison of the antibody detection results between the offspring's serum and colostrum showed a kappa agreement of 1.000. In conclusion, there was a good agreement regarding the detection of anti- IgG between the colostrum samples and the lambs' serum; the use of colostrum forms a noninvasive alternative for diagnosing in sheep herds.
目的是通过间接免疫荧光试验（IFAT）检测母羊血清和初乳样本中抗[具体抗原未提及]IgG抗体的相关性，并评估新生羔羊摄入初乳后血清中这种免疫球蛋白的存在情况。分析了162只在一般体格检查中未显示任何疾病的母羊及其产后不超过5天的新生羔羊的血样，以及162份初乳样本和182份新生儿血样。总体而言，27.8%（45/162）的母羊抗[具体抗原未提及]IgG呈阳性，其中46.7%（21/45）的初乳中检测到抗体。所有初乳呈阳性的母羊所产后代均有反应。母羊血清学检测与初乳结果之间相关性的kappa一致性为0.558。随着母羊抗体滴度的增加，这种相关性增强，当母羊抗体滴度达到1:400时，相关性达到1.000。后代血清与初乳中抗体检测结果的比较显示kappa一致性为1.000。总之，初乳样本与羔羊血清中抗[具体抗原未提及]IgG的检测结果具有良好的一致性；初乳的检测为羊群中[具体疾病未提及]的诊断提供了一种非侵入性的替代方法。
