Amouei Afsaneh, Sharif Mehdi, Sarvi Shahabeddin, Bagheri Nejad Ramin, Aghayan Sargis A, Hashemi-Soteh Mohammad Bagher, Mizani Azadeh, Hosseini Seyed Abdollah, Gholami Sara, Sadeghi Alireza, Sarafrazi Mohammad, Daryani Ahmad
Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Mazandaran, Iran.
Student Research Committee, Mazandaran University of Medical Sciences, Sari, Mazandaran, Iran.
PeerJ. 2019 Jan 18;6:e5920. doi: 10.7717/peerj.5920. eCollection 2019.
In the farming industry, the productivity of livestock herds depends on the fertility efficiency of animals. The accurate diagnosis of a broad range of aetiological agents causing fetal death is often difficult. Our aim was to assess the prevalence rates of , , and spp. infections in ruminant abortion using bacteriological culture and molecular techniques in Mazandaran Province, northern Iran. Samples were collected from 70 aborted sheep, goat, and cattle fetuses between September 2014 and December 2015. Necropsy was performed on all the received samples, and brain tissue and abomasal content were obtained from the aborted fetuses. Protozoan infections were detected by specific polymerase chain reaction (PCR) and bacterial agents using bacteriological examinations and PCR assay. Infectious pathogens were detected in 22 out of 70 (31.4%) examined fetuses. Moreover, , , and were verified in 13 (18.6%), four (5.7%), and two (2.85%) samples, respectively. Our results showed that infection with the mentioned pathogenic agents may lead to fetal mortality, which can be a major cause of economic loss. The listed pathogens could be considered important etiological agents of fetal loss in Mazandaran Province, for which appropriate control measures such as vaccination and biosecurity can be implemented to prevent infection and reduce reproductive loss in livestock farms.
在畜牧业中，畜群的生产力取决于动物的繁殖效率。准确诊断导致胎儿死亡的多种病原体往往很困难。我们的目的是利用细菌培养和分子技术评估伊朗北部马赞德兰省反刍动物流产中 、 和 属感染的患病率。2014年9月至2015年12月期间，从70只流产的绵羊、山羊和牛胎儿中采集样本。对所有收到的样本进行尸检，并从流产胎儿中获取脑组织和皱胃内容物。通过特异性聚合酶链反应（PCR）检测原生动物感染，通过细菌学检查和PCR检测细菌病原体。在70只接受检查的胎儿中，有22只（31.4%）检测到感染性病原体。此外，分别在13份（18.6%）、4份（5.7%）和2份（2.85%）样本中检测到 、 和 。我们的结果表明，感染上述病原体可能导致胎儿死亡，这可能是经济损失的主要原因。所列病原体可被视为马赞德兰省胎儿损失的重要病原体，为此可实施适当的控制措施，如疫苗接种和生物安全措施，以预防感染并减少畜牧场的繁殖损失。
