Cavellier J F, Berry J P, Lagrue G
Histochemistry. 1978 Sep 28;57(4):313-22. doi: 10.1007/BF00492667.
For studies on cathodoluminescence, we equipped a scanning electron microscope with a prism spectrometer and sensitive photomultiplier. The apparatus is described and our initial results are presented on the analyse of cathodoluminescence. The material observed promarily involved studies of immunofluorescent specimens. Humal lymphocytes were labelled with a fluorescent antibody and cryosections of rat kidney with Masugi nephritis were labelled with a fluorescent specific antibody. Our apparatus permitted monochromatic imaging of cathodoluminescence emissions and resulted in much improved micrographs. Some possible improvements of the technique are discussed.
对于阴极发光研究，我们为扫描电子显微镜配备了棱镜光谱仪和灵敏的光电倍增管。本文描述了该仪器，并展示了我们对阴极发光分析的初步结果。观察的材料主要涉及免疫荧光标本的研究。人类淋巴细胞用荧光抗体标记，患有马杉肾炎的大鼠肾脏冷冻切片用荧光特异性抗体标记。我们的仪器可以对阴极发光发射进行单色成像，从而得到质量大为提高的显微照片。本文还讨论了该技术一些可能的改进。
