Schmidt E H, Bröcker W, Wagner H, Pfefferkorn G, Beller F K
Am J Pathol. 1975 Oct;81(1):43-8.
Fluorescein-labeled antigen-antibody complexes could be made visible by scanning electron microscopy using an intensifying device. This new method of immunocathodoluminescence was demonstrated on cryostat sections of rat kidneys containing glomerular fibrin as the result of endotoxin infusion. The resulting photographs correspond with those obtained by immunofluorescent microscopy. The advantage of this technique is, however, the larger depth of focus. By using thinner cyostat sections it is expected that the higher resolution of scanning microscopy will provide even better details in three dimensions.
使用增强装置，通过扫描电子显微镜可使荧光素标记的抗原-抗体复合物变得可见。这种免疫阴极发光的新方法在含有因内毒素注入而产生的肾小球纤维蛋白的大鼠肾脏冷冻切片上得到了验证。所得照片与通过免疫荧光显微镜获得的照片一致。然而，这项技术的优点是焦深更大。通过使用更薄的冷冻切片，预计扫描显微镜的更高分辨率将在三维上提供更好的细节。
